MGI phenotypes (allele matching)
STOCK Rab27btm1.2Seab/SeabH
| Status | Available to order |
| EMMA ID | EM:07825 |
| International strain name | STOCK Rab27btm1.2Seab/SeabH |
| Alternative name | Rab27B knock-out |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Rab27btm1.2Seab, |
| Gene/Transgene symbol | Rab27b |
Information from provider
| Provider | Miguel Seabra |
| Provider affiliation | Molecular Medicine Section, National heart & Lung Institute, Imperial College London |
| Genetic information | Fragment containing exon 2 and 3 of Rab27b gene and adjacent intron sequences was deleted and replaced by loxP site |
| Phenotypic information | Homozygous:Rab27b knock-out exhibit significant hemorrhagic disease. Rab27B KO platelets demonstrated impaired aggregation with collagen and the U46619 thromboxane A2 analogue and reduced secretion of dense granules. Number of dense granules per platelet and platelet serotonin content were reduced in Rab27B knock-out mice.Heterozygous:--- |
| Breeding history | The animals were strictly intercrossed for at least 20 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
Literature references
- Rab27b regulates number and secretion of platelet dense granules.;Tolmachova Tanya, Abrink Magnus, Futter Clare E, Authi Kalwant S, Seabra Miguel C, ;2007;Proceedings of the National Academy of Sciences of the United States of America;104;5872-7; 17384153
- Rab27b regulates mast cell granule dynamics and secretion.;Mizuno Kouichi, Tolmachova Tanya, Ushakov Dmitry S, Romao Maryse, Abrink Magnus, Ferenczi Michael A, Raposo Graça, Seabra Miguel C, ;2007;Traffic (Copenhagen, Denmark);8;883-92; 17587407
- Chromosomal mapping, gene structure and characterization of the human and murine RAB27B gene.;Ramalho J S, Tolmachova T, Hume A N, McGuigan A, Gregory-Evans C Y, Huxley C, Seabra M C, ;2001;BMC genetics;2;2; 11178108
- Rab27b regulates exocytosis of secretory vesicles in acinar epithelial cells from the lacrimal gland.;Chiang Lilian, Ngo Julie, Schechter Joel E, Karvar Serhan, Tolmachova Tanya, Seabra Miguel C, Hume Alistair N, Hamm-Alvarez Sarah F, ;2011;American journal of physiology. Cell physiology;301;C507-21; 21525430