STOCK Gt(ROSA)26Sortm1Hjf Tg(Ltf-icre)14Mmul/Biat
| Status | Available to order |
| EMMA ID | EM:08181 |
| International strain name | STOCK Gt(ROSA)26Sortm1Hjf Tg(Ltf-icre)14Mmul/Biat |
| Alternative name | B6;129-R26tdRFPfl-Tg(Ltf-Cre)14 |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Gt(ROSA)26Sortm1Hjf, |
| Gene/Transgene symbol | Gt(ROSA)26Sor |
Information from provider
| Provider | Dieter Fink |
| Provider affiliation | Institute of Animal Breeding and Genetics, University of Veterinary Medicine Vienna |
| Additional owner | Boris Kovacic, Meduni Wien, Institut fuer Medizinische Genetik, Vienna, Austria |
| Genetic information | The cre-inducible tdRFP cassette was targeted into the Gt(ROSA)26Sor locus of Bruce4 ES cells, which are derived from mice of the widely used inbred C57BL/6 strain. Insertion of a codon-improved cre (icre) recombinase into a bacterial artificial chromosome carrying the Ltf gene (BAC; RP24-166N8; purchased from Children's Hospital Oakland Research Institute, Oakland, CA) was performed via homologous recombination in E. coli. A cassette containing the icre recombinase, an artificial intron, a bovine growth hormone polyadenylation signal and an ampicillin-resistance gene flanked by Frt sites, was recombined into the first exon of the Ltf gene. To delete the ampicillin gene, a plasmid expressing the FLP recombinase was transformed into E.coli harboring the recombined BAC. Correct insertion and ampR-gene deletion were verified by Southern blotting and by DNA sequencing. BAC was purified using a CsCl-gradient and ultracentrifugation and injected into the pro-nuclei of (B6 x 129/Sv)F1 oocytes. |
| Phenotypic information | Homozygous:RFP mice show red fluorescence; Ltf-cre: no homozygous mice produced.Heterozygous:No overt phenotypes in gross morphology, life expectancy or breeding efficiency, nor did we observe any organ pathologies during a breeding period of >36 months |
| Breeding history | Animals with suited genotype were selected, independent if they were sibs or not. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
Literature references
- Lactotransferrin-Cre reporter mice trace neutrophils, monocytes/macrophages and distinct subtypes of dendritic cells.;Kovacic Boris, Hoelbl-Kovacic Andrea, Fischhuber Katrin M, Leitner Nicole R, Gotthardt Dagmar, Casanova Emilio, Sexl Veronika, Müller Mathias, ;2014;Haematologica;99;1006-15; 24561791