C.Cg-Tg(Ltf-icre)14Mmul/Biat
| Status | Available to order |
| EMMA ID | EM:08182 |
| International strain name | C.Cg-Tg(Ltf-icre)14Mmul/Biat |
| Alternative name | C.Cg-Tg(Ltf-Cre)14 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Ltf-icre)14Mmul, |
| Gene/Transgene symbol | Tg(Ltf-icre)14Mmul |
Information from provider
| Provider | Dieter Fink |
| Provider affiliation | Institute of Animal Breeding and Genetics, University of Veterinary Medicine Vienna |
| Additional owner | Boris Kovacic, Meduni Wien, Institut fuer Medizinische Genetik, Vienna, Austria |
| Genetic information | A cassette containing the iCre recombinase, an artificial intron, a bovine growth hormone polyadenylation signal and an ampicillin-resistance gene flanked by Frt (Flp recombinase target) sites, was recombined into the first exon of the Ltf gene. To delete the ampicillin gene, a plasmid expressing the FLP recombinase was transformed into E.coli harboring the recombined BAC. Correct insertion and ampR-gene deletion were verified by Southern blotting and by DNA sequencing. BAC was purified using a CsCl gradient and ultracentrifugation and injected into the pro-nuclei of (B6 x 129/Sv)F1 oocytes. |
| Phenotypic information | Homozygous:no phenotypeHeterozygous:no phenotype |
| References |
|
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
| Animals used for archiving | heterozygous BALB/c |
Literature references
- Lactotransferrin-Cre reporter mice trace neutrophils, monocytes/macrophages and distinct subtypes of dendritic cells.;Kovacic Boris, Hoelbl-Kovacic Andrea, Fischhuber Katrin M, Leitner Nicole R, Gotthardt Dagmar, Casanova Emilio, Sexl Veronika, Müller Mathias, ;2014;Haematologica;99;1006-15; 24561791