129P2/OlaHsd-Gal^tm1Wyn/WynH

Status	Available to order
EMMA ID	EM:08194
International strain name	129P2/OlaHsd-Gal^tm1Wyn/WynH
Alternative name	Galanin KO
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Gal^tm1Wyn,
Gene/Transgene symbol	Gal

Information from provider

Provider	David Wynick
Provider affiliation	Medical Sciences Bristol, Research NBT and UHBristol
Genetic information	Loss of function mutation in the galanin gene.
Phenotypic information	Homozygous: Developmental deficits in dorsal root ganglion (DRG) and cholinergic neurons, absence of neuropathic pain, deficits in neuronal regeneration and neurite outgrowth and more severe disease in experimental autoimmune encephalomyelitis (EAE) model of multiple sclerosis (MS) and A-beta model of Alzheimer's disease (AD). Heterozygous: No phenotype.
Breeding history	E14 ES cell line - maintained on pure 129P2/OlaHsd
References	A role for galanin in human and experimental inflammatory demyelination.;Wraith David C, Pope Robert, Butzkueven Helmut, Holder Heidi, Vanderplank Penny, Lowrey Pauline, Day Michael J, Gundlach Andrew L, Kilpatrick Trevor J, Scolding Neil, Wynick David, ;2009;Proceedings of the National Academy of Sciences of the United States of America;106;15466-71; 19717462 Feeding behaviour in galanin knockout mice supports a role of galanin in fat intake and preference.;Adams A C, Clapham J C, Wynick D, Speakman J R, ;2008;Journal of neuroendocrinology;20;199-206; 18088361 Loss-of-function mutation of the galanin gene is associated with perturbed islet function in mice.;Ahrén Bo, Pacini Giovanni, Wynick David, Wierup Nils, Sundler Frank, ;2004;Endocrinology;145;3190-6; 15044363 Galanin acts as a neuroprotective factor to the hippocampus.;Elliott-Hunt Caroline R, Marsh Barnaby, Bacon Andrea, Pope Robert, Vanderplank Penny, Wynick David, ;2004;Proceedings of the National Academy of Sciences of the United States of America;101;5105-10; 15041741 The second galanin receptor GalR2 plays a key role in neurite outgrowth from adult sensory neurons.;Mahoney Sally-Ann, Hosking Richard, Farrant Sarah, Holmes Fiona E, Jacoby Arie S, Shine John, Iismaa Tiina P, Scott Malcolm K, Schmidt Ralf, Wynick David, ;2003;The Journal of neuroscience : the official journal of the Society for Neuroscience;23;416-21; 12533601 Endogenous galanin is required for the full expression of central sensitization following peripheral nerve injury.;Kerr B J, Thompson S W, Wynick D, McMahon S B, ;2001;Neuroreport;12;3331-4; 11711881 Targeted disruption of the galanin gene reduces the number of sensory neurons and their regenerative capacity.;Holmes F E, Mahoney S, King V R, Bacon A, Kerr N C, Pachnis V, Curtis R, Priestley J V, Wynick D, ;2000;Proceedings of the National Academy of Sciences of the United States of America;97;11563-8; 11016970 Galanin knockout mice reveal nociceptive deficits following peripheral nerve injury.;Kerr B J, Cafferty W B, Gupta Y K, Bacon A, Wynick D, McMahon S B, Thompson S W, ;2000;The European journal of neuroscience;12;793-802; 10762308 Modulation of hippocampal excitability and seizures by galanin.;Mazarati A M, Hohmann J G, Bacon A, Liu H, Sankar R, Steiner R A, Wynick D, Wasterlain C G, ;2000;The Journal of neuroscience : the official journal of the Society for Neuroscience;20;6276-81; 10934278 Galanin regulates the postnatal survival of a subset of basal forebrain cholinergic neurons.;O'Meara G, Coumis U, Ma S Y, Kehr J, Mahoney S, Bacon A, Allen S J, Holmes F, Kahl U, Wang F H, Kearns I R, Ove-Ogren S, Dawbarn D, Mufson E J, Davies C, Dawson G, Wynick D, ;2000;Proceedings of the National Academy of Sciences of the United States of America;97;11569-74; 11016971
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

MGI phenotypes (allele matching)

abnormal branching of the mammary ductal tree / MGI
abnormal hippocampus morphology / MGI
decreased sensory neuron number / MGI
small L4 dorsal root ganglion / MGI
small L5 dorsal root ganglion / MGI
abnormal object recognition memory / MGI
abnormal circulating insulin level / MGI
abnormal pituitary secretion / MGI
abnormal lactation / MGI
seizures / MGI
abnormal gland morphology / MGI
abnormal gland physiology / MGI
increased susceptibility to pharmacologically induced seizures / MGI
enhanced long term potentiation / MGI
decreased insulin secretion / MGI
abnormal nervous system regeneration / MGI
increased susceptibility to neuronal excitotoxicity / MGI
decreased lactotroph cell number / MGI
lactation failure / MGI
weight loss / MGI
abnormal testes secretion / MGI
enlarged seminal vesicle / MGI
decreased circulating glucocorticoid level / MGI
decreased testis weight / MGI

Literature references

A role for galanin in human and experimental inflammatory demyelination.;Wraith David C, Pope Robert, Butzkueven Helmut, Holder Heidi, Vanderplank Penny, Lowrey Pauline, Day Michael J, Gundlach Andrew L, Kilpatrick Trevor J, Scolding Neil, Wynick David, ;2009;Proceedings of the National Academy of Sciences of the United States of America;106;15466-71; 19717462
Feeding behaviour in galanin knockout mice supports a role of galanin in fat intake and preference.;Adams A C, Clapham J C, Wynick D, Speakman J R, ;2008;Journal of neuroendocrinology;20;199-206; 18088361
Loss-of-function mutation of the galanin gene is associated with perturbed islet function in mice.;Ahrén Bo, Pacini Giovanni, Wynick David, Wierup Nils, Sundler Frank, ;2004;Endocrinology;145;3190-6; 15044363
Galanin acts as a neuroprotective factor to the hippocampus.;Elliott-Hunt Caroline R, Marsh Barnaby, Bacon Andrea, Pope Robert, Vanderplank Penny, Wynick David, ;2004;Proceedings of the National Academy of Sciences of the United States of America;101;5105-10; 15041741
The second galanin receptor GalR2 plays a key role in neurite outgrowth from adult sensory neurons.;Mahoney Sally-Ann, Hosking Richard, Farrant Sarah, Holmes Fiona E, Jacoby Arie S, Shine John, Iismaa Tiina P, Scott Malcolm K, Schmidt Ralf, Wynick David, ;2003;The Journal of neuroscience : the official journal of the Society for Neuroscience;23;416-21; 12533601
Endogenous galanin is required for the full expression of central sensitization following peripheral nerve injury.;Kerr B J, Thompson S W, Wynick D, McMahon S B, ;2001;Neuroreport;12;3331-4; 11711881
Targeted disruption of the galanin gene reduces the number of sensory neurons and their regenerative capacity.;Holmes F E, Mahoney S, King V R, Bacon A, Kerr N C, Pachnis V, Curtis R, Priestley J V, Wynick D, ;2000;Proceedings of the National Academy of Sciences of the United States of America;97;11563-8; 11016970
Galanin knockout mice reveal nociceptive deficits following peripheral nerve injury.;Kerr B J, Cafferty W B, Gupta Y K, Bacon A, Wynick D, McMahon S B, Thompson S W, ;2000;The European journal of neuroscience;12;793-802; 10762308
Modulation of hippocampal excitability and seizures by galanin.;Mazarati A M, Hohmann J G, Bacon A, Liu H, Sankar R, Steiner R A, Wynick D, Wasterlain C G, ;2000;The Journal of neuroscience : the official journal of the Society for Neuroscience;20;6276-81; 10934278
Galanin regulates the postnatal survival of a subset of basal forebrain cholinergic neurons.;O'Meara G, Coumis U, Ma S Y, Kehr J, Mahoney S, Bacon A, Allen S J, Holmes F, Kahl U, Wang F H, Kearns I R, Ove-Ogren S, Dawbarn D, Mufson E J, Davies C, Dawson G, Wynick D, ;2000;Proceedings of the National Academy of Sciences of the United States of America;97;11569-74; 11016971

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129P2/OlaHsd-Galtm1Wyn/WynH