MGI phenotypes (allele matching)
B6.129P2-Ptcratm1(icre)Hjf/Ieg
| Status | Available to order |
| EMMA ID | EM:08347 |
| International strain name | B6.129P2-Ptcratm1(icre)Hjf/Ieg |
| Alternative name | pTa-iCre knock-in |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Ptcratm1(icre)Hjf, |
| Gene/Transgene symbol | Ptcra |
Information from provider
| Provider | Hans Joerg Fehling |
| Provider affiliation | University Clinics Ulm, Institute for Immunology |
| Genetic information | A mini-gene carrying a codon-optimized version of Cre recombinase (iCre) has been inserted by homologous recombination into the first exon of the Ptcra gene encoding the Pre-T-cell receptor alpha protein. The knock-in inactivates the targeted Ptcra allele. |
| Phenotypic information | Homozygous:Mice show same defects as classical Ptcra knock-out mice: partial developmental block at the transition from DN3 to DN4 stage resulting in impaired thymopoiesis of ab T cells.Heterozygous:Loss of one functional Ptcra allele; no apparent defects |
| Breeding history | The knock-in was produced in E14.1 ES cells derived from 129/Ola mice. Chimaeras were intercrossed with C57BL/6 breeding partners. Mice carrying the pTa-iCre knock-in allele were backcrossed for 20 generations onto C57BL/6. At 20th generation backcross mice were made homozygous for the knock-in allele by intercrossing heterozygous mating partners. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | homozygous C57BL/6, wild-type C57BL/6J |
| Stage of embryos | 2-cell |
Disease and phenotype information
Literature references
- In vivo fate mapping identifies pre-TCRα expression as an intra- and extrathymic, but not prethymic, marker of T lymphopoiesis.;Luche Hervé, Nageswara Rao Tata, Kumar Suresh, Tasdogan Alpaslan, Beckel Franziska, Blum Carmen, Martins Vera C, Rodewald Hans-Reimer, Fehling Hans Jörg, ;2013;The Journal of experimental medicine;210;699-714; 23509324