B6.129(C3)-Rab27aash Rab27btm1.2Seab/SeabH

Status

Available to order

EMMA IDEM:08549
International strain nameB6.129(C3)-Rab27aash Rab27btm1.2Seab/SeabH
Alternative nameRab27a Rab27b double knock-out (Rab27a ash/ash Rab27b null/null)
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolRab27btm1.2Seab,
Gene/Transgene symbolRab27b

Information from provider

ProviderMiguel Seabra
Provider affiliationMolecular Medicine Section, National Heart , Imperial College London
Additional ownerProf. Miguel Seabra and Dr. Tanya Tolmachova, Imperial College, London, UK
Genetic informationStrain is homozygous for Rab27aash mutation and Rab27b null allele
Phenotypic informationHomozygous:
Abnormal coat colour pigmentation (ashen) due to Rab27a mutation

Heterozygous:
normal
Breeding historyRab27aash arose spontaneously on a C3H/He genetic background. Rab27btm1.2Seab was a targeted mutation produced on a GSI-1 embryonic cell line of 129X1/SvJ origin. The stock carrying both mutations was outcrossed to C57BL/6 (backcrossing for 10 generations), followed by sib mating for a further 10 generations.
References
  • Rab27b regulates number and secretion of platelet dense granules.;Tolmachova Tanya, Abrink Magnus, Futter Clare E, Authi Kalwant S, Seabra Miguel C, ;2007;Proceedings of the National Academy of Sciences of the United States of America;104;5872-7; 17384153
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

MGI allele-associated human disease models

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • abnormal platelet dense granule morphology / MGI
  • decreased platelet serotonin level / MGI
  • increased bleeding time / MGI
  • abnormal platelet dense granule physiology / MGI
  • decreased platelet ATP level / MGI
  • decreased platelet aggregation / MGI
  • decreased platelet ADP level / MGI
  • decreased platelet dense granule number / MGI
  • diluted coat color / MGI
  • abnormal coat/hair pigmentation / MGI
  • abnormal skin pigmentation / MGI
  • abnormal melanocyte morphology / MGI
  • abnormal Harderian gland pigmentation / MGI
  • impaired natural killer cell mediated cytotoxicity / MGI
  • decreased cytotoxic T cell cytolysis / MGI
  • immune system phenotype / MGI
  • vision/eye phenotype / MGI
  • abnormal vesicle-mediated transport / MGI
  • abnormal NK cell degranulation / MGI
  • abnormal melanosome transport / MGI
  • integument phenotype / MGI
  • decreased leukocyte cell number / MGI
  • decreased neutrophil cell number / MGI
  • weight loss / MGI
  • hunched posture / MGI
  • increased susceptibility to viral infection / MGI
  • decreased erythrocyte cell number / MGI
  • thrombocytopenia / MGI
  • abnormal cytotoxic T cell physiology / MGI
  • pancytopenia / MGI
  • lethargy / MGI
  • increased circulating aspartate transaminase level / MGI
  • decreased body temperature / MGI
  • increased circulating tumor necrosis factor level / MGI
  • increased circulating interferon-gamma level / MGI
  • increased circulating interleukin-6 level / MGI
  • increased circulating interleukin-1 beta level / MGI
  • decreased NK cell degranulation / MGI
  • increased circulating lactate dehydrogenase level / MGI
  • abnormal blood coagulation / MGI

Literature references

  • Rab27b regulates number and secretion of platelet dense granules.;Tolmachova Tanya, Abrink Magnus, Futter Clare E, Authi Kalwant S, Seabra Miguel C, ;2007;Proceedings of the National Academy of Sciences of the United States of America;104;5872-7; 17384153

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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