C57BL/6-Gata6tm1(EGFP/cre/ERT2)Fmw/H
| Status | Available to order |
| EMMA ID | EM:08976 |
| International strain name | C57BL/6-Gata6tm1(EGFP/cre/ERT2)Fmw/H |
| Alternative name | Gata6cre (C57BL/6J) |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Gata6tm1(EGFP/cre/ERT2)Fmw, |
| Gene/Transgene symbol | Gata6 |
Information from provider
| Provider | Fiona Watt |
| Provider affiliation | Centre for Stem Cell and Regenerative medicine, King |
| Genetic information | Tamoxifen-inducible cre recombinase is expressed under endogenous Gata6 promoter. For the generation of the Gata6 eGFP-CreERT2 knock-in construct, an IKMC intermediate vector targeted on C57BL/6J BACs was used. A final targeting vector was constructed using gateway modular strategy as described in Skarnes et al. (Nature, 2011). The final vector was then electroplated into JM8 ES cells and selected for puromycin antibiotic resistance. Allelic targeting was determined in single clones by restriction digestion and sequencing of genomic DNA. The copy number was determined by qPCR probe against puromycin sequence. The final targeting allele would result in an eGFP-CreERT2 expression element targeted in-frame with the first non-coding exon of Gata6 gene and further frameshifting (Gata6-eGFPCreERT2). The mouse mutant has been generated to perform lineage tracing experiments or to create inducible conditional transgenic with floxed constructs. These allow to study Gata6 progeny. This gene is widely expressed and an inducible construct like this was not available yet. For this reason many other scientists will be interested on this new strain. Gata6 is an essential gene for embryonic development. This mouse line has to be maintained heterozygous because the CreER transgenic cassette we used leads to genetic loss of Gata6. |
| Phenotypic information | Homozygous:LethalHeterozygous:Viable |
| Breeding history | New strain recently generated in MRC Harwell. |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
| Animals used for archiving | heterozygous C57BL/6J |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Familial atrial fibrillation / Orphanet_334
- Pancreatic hypoplasia-diabetes-congenital heart disease syndrome / Orphanet_2255
- Complete atrioventricular septal defect with ventricular hypoplasia / Orphanet_99067
- Atrial septal defect, ostium secundum type / Orphanet_99103
Literature references
- Wounding induces dedifferentiation of epidermal Gata6+ cells and acquisition of stem cell properties.;Donati Giacomo, Rognoni Emanuel, Hiratsuka Toru, Liakath-Ali Kifayathullah, Hoste Esther, Kar Gozde, Kayikci Melis, Russell Roslin, Kretzschmar Kai, Mulder Klaas W, Teichmann Sarah A, Watt Fiona M, ;2017;Nature cell biology;19;603-613; 28504705