B6;D-Tg(tetO-GFP,-lacZ)G3Rsp/Kctt
| Status | Available to order |
| EMMA ID | EM:09285 |
| International strain name | B6;D-Tg(tetO-GFP,-lacZ)G3Rsp/Kctt |
| Alternative name | TgTetO/GFP-lacZ (lab name B6xG3 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(tetO-GFP, -lacZ)G3Rsp |
| Gene/Transgene symbol | Tg(tetO-GFP,-lacZ)G3Rsp |
Information from provider
| Provider | Rolf Sprengel |
| Provider affiliation | Molecular Neurobiology, Max Planck Institute for Medical Research |
| Genetic information | A transgene for enhanced GFP together with a lacZ reporter gene is under the control of the tTA dependent bidirectional synthetic promoter. |
| Phenotypic information | Homozygous:Not knownHeterozygous:When the GFP and lacZ transgene is activated by tTA, the cells that express tTA can be visualised by GFP fluorescence or beta-galactosidase activity. |
| Breeding history | Embryos from mating hemizygous transgenic TgTetO/GFP-lacZ males and C57BL/6N females were harvested and 251 embryos were cryoconserved in January 2003. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | wild-type C57BL/6N |
| Stage of embryos | 2-cell |
Literature references
- A GFP-equipped bidirectional expression module well suited for monitoring tetracycline-regulated gene expression in mouse.;Krestel H E, Mayford M, Seeburg P H, Sprengel R, ;2001;Nucleic acids research;29;E39; 11266574
- Neuronal co-expression of EGFP and beta-galactosidase in mice causes neuropathology and premature death.;Krestel Heinz E, Mihaljevic André L A, Hoffman Dax A, Schneider Armin, ;2004;Neurobiology of disease;17;310-8; 15474368