MGI phenotypes (allele matching)
- no phenotypic analysis / MGI
STOCK Gt(ROSA)26Sortm2(ECFP)Cos/H
| Status | Available to order |
| EMMA ID | EM:09667 |
| International strain name | STOCK Gt(ROSA)26Sortm2(ECFP)Cos/H |
| Alternative name | Gt(ROSA)26Sor |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Gt(ROSA)26Sortm2(ECFP)Cos, |
| Gene/Transgene symbol | Gt(ROSA)26Sor |
Information from provider
| Provider | Shankar Srinivas |
| Provider affiliation | DPAG, University of Oxford |
| Genetic information | The Gt(ROSA)26Sor locus is expressed in practically all mouse tissues, in embryos, neonates, adults. Originally discovered by insertion of a gene-trap vector with a lacZ reporter gene (Friedrich and Soriano, 1991). ES cells and blastocysts derived from this line, (commonly called ROSA26-lacZ) have been used in innumerable developmental studies, to mark and follow specific lineages of cells. A cre-reporter line called R26R was published in 1999, taking advantage of the ubiquitous expression of the Gt(ROSA)26Sor locus (Soriano et al., 1999). The lacZ reporter gene is not expressed unless the cell also expresses (or has expressed in the past) the cre recombinase protein. This line has been used in many publications, to determine where cre is active in cre-expressing transgenic mice. This variant of the R26R reporter mice has been engineered, where, instead of lacZ, ECFP (enhanced cyan fluorescent protein) is expressed as the reporter gene (Srinivas et al., 2001). |
| Phenotypic information | Homozygous:None visible. Have the potential to express ECFP in any tissue, upon cre recombinase-mediated activation.Heterozygous:None visible. Have the potential to express ECFP in any tissue, upon cre recombinase-mediated activation. |
| Breeding history | Mixed background of CBA/Ca x C57BL/10ScSn and CBA/Ca x C57BL/6JNimr. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
Literature references
- Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus.;Srinivas S, Watanabe T, Lin C S, William C M, Tanabe Y, Jessell T M, Costantini F, ;2001;BMC developmental biology;1;4; 11299042