MGI phenotypes (allele matching)
B6.129S2-Msx1tm2.1(cre/ERT2)Bero/Kctt
| Status | Available to order |
| EMMA ID | EM:09717 |
| International strain name | B6.129S2-Msx1tm2.1(cre/ERT2)Bero/Kctt |
| Alternative name | Msx1tm2.1(cre/ERT2)Bero (Synonym : Msx1-CreERT2) |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Msx1tm2.1(cre/ERT2)Bero, |
| Gene/Transgene symbol | Msx1 |
Information from provider
| Provider | Benoit Robert |
| Provider affiliation | Developmental and Stem Cell Biology, Institut Pasteur |
| Genetic information | Insertion by homologous recombination of the complete CreERT2 coding sequence at the ATG start codon of the Msx1 locus. The result allele expresses the CreERT2 fusion protein instead of the genuine Msx1 protein. The CreERT2 protein displays a inducible version of the Cre recombinase, which activity can be induced by injection of Tamoxifen. |
| Phenotypic information | Homozygous:The Msx1tm2.1(cre/ERT2)Bero (Synonym: Msx1-CreERT2) mutation is a null Msx1 mutation. The main phenotype for the homozygotes is the presence at birth of a cleft palate that induces death during the first 24 hours due to the impossibility for the new-born to feed correctly. In addition, these animals display tooth agenesis and, in the brain, the absence of the sub-commissural organ that may lead to hydrocephaly.Heterozygous:Heterozygotes do not display any abnormality except, at a low frequency, hydrocephaly. |
| Breeding history | 14 backcrosses to C57BL/6 |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | heterozygous C57BL/6J |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Hypodontia-dysplasia of nails syndrome / Orphanet_2228
- Oligodontia / Orphanet_99798
Literature references
- Generation and characterization of a tamoxifen inducible Msx1(CreERT2) knock-in allele.;Lallemand Yvan, Moreau Julie, Cloment Cécile Saint, Vives Francina Langa, Robert Benoît, ;2013;Genesis (New York, N.Y. : 2000);51;110-9; 23090744