B6.129-Col13a1tm3.1Pih/Oulu

Status

Available to order

EMMA IDEM:09878
International strain nameB6.129-Col13a1tm3.1Pih/Oulu
Alternative nameB6.129-Col13a1
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolCol13a1tm3.1Pih,
Gene/Transgene symbolCol13a1

Information from provider

ProviderTaina Pihlajaniemi
Provider affiliationFaculty of Biochemistry and Molecular medicine, University of Oulu
Genetic informationExon 1, the first protein-coding exon, and flanking sequences were replaced with a floxed neo cassette; cre-mediated recombination removed the neo cassette leaving behind one loxP site to replace exon 1 and flanking sequences.
Phenotypic informationHomozygous:
Behavior/neurological: tremors. Growth/size/body: postnatal growth retardation. Nervous system: abnormal neuromuscular synapse morphology, abnormal synaptic vesicle morphology, abnormal endplate potential, abnormal miniature endplate potential, abnormal synaptic acetylcholine release.

Heterozygous:
Slightly abnormal neuromuscular synapse morphology.
Breeding historyES cell line used was R1. Only one correctly targeted clone generated chimeras. Mice were crossed with Tg(CAG-cre)13Miya mice to exclude loxP-flanked neo cassette from the genome. Mice were backcrossed for 16 generations with C57BL/6JOlaHsd mice.
References
  • Muscle-derived collagen XIII regulates maturation of the skeletal neuromuscular junction.;Latvanlehto Anne, Fox Michael A, Sormunen Raija, Tu Hongmin, Oikarainen Tuomo, Koski Anu, Naumenko Nikolay, Shakirzyanova Anastasia, Kallio Mika, Ilves Mika, Giniatullin Rashid, Sanes Joshua R, Pihlajaniemi Taina, ;2010;The Journal of neuroscience : the official journal of the Society for Neuroscience;30;12230-41; 20844119
  • Collagen XIII secures pre- and postsynaptic integrity of the neuromuscular synapse.;Härönen Heli, Zainul Zarin, Tu Hongmin, Naumenko Nikolay, Sormunen Raija, Miinalainen Ilkka, Shakirzyanova Anastasia, Oikarainen Tuomo, Abdullin Azat, Martin Paula, Santoleri Sabrina, Koistinaho Jari, Silman Israel, Giniatullin Rashid, Fox Michael A, Heikkinen Anne, Pihlajaniemi Taina, ;2017;Human molecular genetics;26;2076-2090; 28369367
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreUniversity of Oulu, Oulu, Finland
Animals used for archivinghomozygous C57BL/6JOlaHsd, wild-type C57BL/6JOlaHsd
Stage of embryos2-cell

Disease and phenotype information

MGI allele-associated human disease models

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • tremors / MGI
  • abnormal neuromuscular synapse morphology / MGI
  • postnatal growth retardation / MGI
  • abnormal endplate potential / MGI
  • abnormal synaptic vesicle morphology / MGI
  • abnormal miniature endplate potential / MGI
  • abnormal synaptic acetylcholine release / MGI

Literature references

  • Muscle-derived collagen XIII regulates maturation of the skeletal neuromuscular junction.;Latvanlehto Anne, Fox Michael A, Sormunen Raija, Tu Hongmin, Oikarainen Tuomo, Koski Anu, Naumenko Nikolay, Shakirzyanova Anastasia, Kallio Mika, Ilves Mika, Giniatullin Rashid, Sanes Joshua R, Pihlajaniemi Taina, ;2010;The Journal of neuroscience : the official journal of the Society for Neuroscience;30;12230-41; 20844119
  • Collagen XIII secures pre- and postsynaptic integrity of the neuromuscular synapse.;Härönen Heli, Zainul Zarin, Tu Hongmin, Naumenko Nikolay, Sormunen Raija, Miinalainen Ilkka, Shakirzyanova Anastasia, Oikarainen Tuomo, Abdullin Azat, Martin Paula, Santoleri Sabrina, Koistinaho Jari, Silman Israel, Giniatullin Rashid, Fox Michael A, Heikkinen Anne, Pihlajaniemi Taina, ;2017;Human molecular genetics;26;2076-2090; 28369367

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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