CByIco.129-Rag2tm1Fwa Cd44tm1.1Ugu/H

Status

Available to order

EMMA IDEM:02022
International strain nameCByIco.129-Rag2tm1Fwa Cd44tm1.1Ugu/H
Alternative nameBALB/cByJIco Rag2-/- CD44v6v7-/-
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolCd44tm1.1Ugu, Rag2tm1Fwa
Gene/Transgene symbolCd44, Rag2

Information from provider

ProviderUrsula Günthert
Provider affiliationInstitut für Pathologie, University of Basel
Genetic informationA loxP site was inserted 3' of exon v6 and a neomycin resistance cassette, with a 5' loxP site, was inserted 5' of exon v6 of the Cd44 gene. During insertion, 1.6 kb of sequence was lost in the exon v7 region; cre recombinase was transiently expressed in ES cells allowing the removal of neomycin cassette and exon v6. Mice lack both exon v6 and v7. Insertion of a neomycin resistance cassette into the unique exon of the Rag2 gene by homologous recombination leading to the deletion of 286 amino acids.
Phenotypic informationRag2-/-: No mature T and B cells. Cd44v6v7-/-: Strongly reduced symptoms in autoimmune diseases.
References
  • RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.;Shinkai Y, Rathbun G, Lam K P, Oltz E M, Stewart V, Mendelsohn M, Charron J, Datta M, Young F, Stall A M, ;1992;Cell;68;855-67; 1547487
  • Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archivinghomozygous BALB/cByJIco, homozygous BALB/cByJIco
Breeding at archiving centreMaintained by intercrossing double homozygotes prior to and during archiving
Stage of embryos2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
  • decreased T cell proliferation / MGI
  • abnormal digestive system physiology / MGI
  • immune system phenotype / MGI
  • abnormal T-helper 1 physiology / MGI
  • abnormal T-helper 2 physiology / MGI
  • increased granulocyte number / MGI
  • abnormal spleen morphology / MGI
  • spleen hypoplasia / MGI
  • decreased body size / MGI
  • arrested B cell differentiation / MGI
  • decreased IgM level / MGI
  • thymus hypoplasia / MGI
  • arrested T cell differentiation / MGI
  • increased susceptibility to infection / MGI
  • abnormal effector T cell morphology / MGI
  • increased natural killer cell mediated cytotoxicity / MGI
  • decreased double-positive T cell number / MGI
  • increased macrophage cell number / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • lymph node hypoplasia / MGI
  • absent mature B cells / MGI
  • abnormal T cell receptor V(D)J recombination / MGI
  • abnormal immunoglobulin V(D)J recombination / MGI
  • decreased thymocyte number / MGI
  • lung inflammation / MGI
  • increased susceptibility to bacterial infection / MGI
  • abnormal macrophage physiology / MGI
  • colitis / MGI
  • abnormal response to transplant / MGI
  • abnormal interferon secretion / MGI
  • abnormal chemokine secretion / MGI
  • decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
  • abnormal intestinal mucosa morphology / MGI
  • abnormal B cell morphology / MGI
  • decreased susceptibility to parasitic infection / MGI
  • decreased double-negative T cell number / MGI
  • increased double-negative T cell number / MGI
  • decreased susceptibility to type IV hypersensitivity reaction / MGI
  • abnormal stomach mucosa morphology / MGI
  • abnormal intestinal epithelium morphology / MGI
  • abnormal B cell number / MGI
  • decreased pre-B cell number / MGI
  • decreased mature B cell number / MGI
  • increased immature B cell number / MGI
  • decreased immature B cell number / MGI
  • abnormal gamma-delta T cell differentiation / MGI
MGI phenotypes (gene matching)
  • decreased mast cell number / MGI
  • altered response to myocardial infarction / MGI
  • abnormal cell morphology / MGI
  • decreased mast cell histamine storage / MGI
  • abnormal uterus morphology / MGI
  • thin epidermis / MGI
  • abnormal lipid level / MGI
  • abnormal vasculogenesis / MGI
  • abnormal digestive system physiology / MGI
  • impaired wound healing / MGI
  • impaired macrophage phagocytosis / MGI
  • liver inflammation / MGI
  • lung inflammation / MGI
  • abnormal definitive hematopoiesis / MGI
  • no abnormal phenotype detected / MGI
  • abnormal CD4-positive, alpha beta T cell morphology / MGI
  • abnormal neutrophil physiology / MGI
  • short tibia / MGI
  • impaired skin barrier function / MGI
  • delayed wound healing / MGI
  • decreased myocardial infarction size / MGI
  • abnormal leukocyte migration / MGI
  • peritoneal inflammation / MGI
  • decreased susceptibility to induced arthritis / MGI
  • decreased tumor growth/size / MGI
  • abnormal leukocyte adhesion / MGI
  • abnormal physiological neovascularization / MGI
  • increased susceptibility to induced arthritis / MGI
  • enhanced coordination / MGI
  • decreased cholesterol level / MGI
  • abnormal vascular endothelial cell physiology / MGI
  • decreased susceptibility to kidney reperfusion injury / MGI
  • abnormal miniature excitatory postsynaptic currents / MGI
  • decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
  • increased mean systemic arterial blood pressure / MGI
  • abnormal CD8-positive, alpha beta T cell morphology / MGI
  • abnormal response to infection / MGI
  • increased susceptibility to parasitic infection / MGI
  • impaired natural killer cell mediated cytotoxicity / MGI
  • decreased acute inflammation / MGI
  • increased acute inflammation / MGI
  • decreased T cell proliferation / MGI
  • digestive/alimentary phenotype / MGI
  • immune system phenotype / MGI
  • hematopoietic system phenotype / MGI
  • abnormal CD4-positive, alpha-beta T cell physiology / MGI
  • abnormal T-helper 1 physiology / MGI
  • abnormal T-helper 2 physiology / MGI
  • choroidal neovascularization / MGI
  • decreased circulating creatinine level / MGI
  • decreased blood urea nitrogen level / MGI
  • abnormal vascular smooth muscle physiology / MGI
  • decreased angiogenesis / MGI
  • decreased susceptibility to type IV hypersensitivity reaction / MGI
  • abnormal cell physiology / MGI
  • abnormal vascular endothelial cell morphology / MGI
  • abnormal involution of the mammary gland / MGI
  • increased diameter of tibia / MGI
  • abnormal osteoclast differentiation / MGI
  • increased circulating tumor necrosis factor level / MGI
  • decreased circulating tumor necrosis factor level / MGI
  • increased tumor necrosis factor secretion / MGI
  • abnormal interleukin secretion / MGI
  • increased circulating interferon-gamma level / MGI
  • increased circulating interleukin-2 level / MGI
  • increased interleukin-1 beta secretion / MGI
  • increased interleukin-10 secretion / MGI
  • increased interleukin-6 secretion / MGI
  • impaired neutrophil recruitment / MGI
  • abnormal chemokine secretion / MGI
  • decreased transforming growth factor level / MGI
  • decreased sensitivity to induced cell death / MGI
  • abnormal epidermal lamellar body morphology / MGI
  • increased sensitivity to induced morbidity/mortality / MGI
  • abnormal circulating chemokine level / MGI
  • abnormal circulating cytokine level / MGI
  • decreased activation-induced cell death of T cells / MGI
  • increased mitochondria size / MGI
  • abnormal mitochondrial crista morphology / MGI
  • decreased fibroblast proliferation / MGI
  • impaired leukocyte tethering or rolling / MGI
  • decreased monocyte cell number / MGI
  • increased granulocyte number / MGI
  • decreased bone marrow cell number / MGI
  • alopecia / MGI
  • abnormal digestive system morphology / MGI
  • abnormal intestinal epithelium morphology / MGI
  • abnormal intestinal mucosa morphology / MGI
  • abnormal spleen morphology / MGI
  • small spleen / MGI
  • spleen hypoplasia / MGI
  • abnormal Peyer's patch morphology / MGI
  • abnormal thymus morphology / MGI
  • decreased thymocyte number / MGI
  • abnormal immune system cell morphology / MGI
  • decreased body size / MGI
  • abnormal humoral immune response / MGI
  • arrested B cell differentiation / MGI
  • decreased IgG level / MGI
  • decreased IgM level / MGI
  • decreased IgA level / MGI
  • thymus hypoplasia / MGI
  • arrested T cell differentiation / MGI
  • lung inflammation / MGI
  • neoplasm / MGI
  • abnormal B cell differentiation / MGI
  • no abnormal phenotype detected / MGI
  • small lymph nodes / MGI
  • abnormal lymph node morphology / MGI
  • abnormal lymphopoiesis / MGI
  • abnormal pre-B cell morphology / MGI
  • increased susceptibility to infection / MGI
  • abnormal double-negative T cell morphology / MGI
  • abnormal double-positive T cell morphology / MGI
  • increased susceptibility to bacterial infection / MGI
  • abnormal effector T cell morphology / MGI
  • abnormal macrophage physiology / MGI
  • abnormal B cell number / MGI
  • abnormal B cell physiology / MGI
  • abnormal immune system organ morphology / MGI
  • colitis / MGI
  • absent Peyer's patches / MGI
  • increased natural killer cell mediated cytotoxicity / MGI
  • erythroderma / MGI
  • abnormal lymphocyte physiology / MGI
  • abnormal B cell morphology / MGI
  • decreased lymphocyte cell number / MGI
  • decreased B cell number / MGI
  • decreased T cell number / MGI
  • decreased susceptibility to parasitic infection / MGI
  • decreased double-negative T cell number / MGI
  • increased double-negative T cell number / MGI
  • decreased double-positive T cell number / MGI
  • decreased T cell proliferation / MGI
  • cachexia / MGI
  • increased macrophage cell number / MGI
  • abnormal T-helper 1 physiology / MGI
  • decreased susceptibility to type IV hypersensitivity reaction / MGI
  • abnormal response to transplant / MGI
  • abnormal T cell morphology / MGI
  • decreased NK T cell number / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • lymph node hypoplasia / MGI
  • decreased pre-B cell number / MGI
  • decreased mature B cell number / MGI
  • absent mature B cells / MGI
  • increased immature B cell number / MGI
  • decreased immature B cell number / MGI
  • abnormal gamma-delta T cell differentiation / MGI
  • decreased spleen white pulp amount / MGI
  • abnormal interferon secretion / MGI
  • abnormal chemokine secretion / MGI
  • abnormal T cell receptor V(D)J recombination / MGI
  • abnormal immunoglobulin V(D)J recombination / MGI
  • absent Hassall's corpuscle / MGI
  • small inguinal lymph nodes / MGI
  • decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
  • increased DN3 thymocyte number / MGI
  • abnormal stomach mucosa morphology / MGI
  • small cervical lymph nodes / MGI

Literature references

  • RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.;Shinkai Y, Rathbun G, Lam K P, Oltz E M, Stewart V, Mendelsohn M, Charron J, Datta M, Young F, Stall A M, ;1992;Cell;68;855-67; 1547487
  • Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330

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Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
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