- decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
- decreased T cell proliferation / MGI
- abnormal digestive system physiology / MGI
- immune system phenotype / MGI
- abnormal T-helper 1 physiology / MGI
- abnormal T-helper 2 physiology / MGI
- increased granulocyte number / MGI
- abnormal spleen morphology / MGI
- spleen hypoplasia / MGI
- decreased body size / MGI
- arrested B cell differentiation / MGI
- decreased IgM level / MGI
- thymus hypoplasia / MGI
- arrested T cell differentiation / MGI
- increased susceptibility to infection / MGI
- abnormal effector T cell morphology / MGI
- increased natural killer cell mediated cytotoxicity / MGI
- decreased double-positive T cell number / MGI
- increased macrophage cell number / MGI
- decreased CD4-positive, alpha beta T cell number / MGI
- decreased CD8-positive, alpha-beta T cell number / MGI
- lymph node hypoplasia / MGI
- absent mature B cells / MGI
- abnormal T cell receptor V(D)J recombination / MGI
- abnormal immunoglobulin V(D)J recombination / MGI
- decreased thymocyte number / MGI
- lung inflammation / MGI
- increased susceptibility to bacterial infection / MGI
- abnormal macrophage physiology / MGI
- colitis / MGI
- abnormal response to transplant / MGI
- abnormal interferon secretion / MGI
- abnormal chemokine secretion / MGI
- decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
- abnormal intestinal mucosa morphology / MGI
- abnormal B cell morphology / MGI
- decreased susceptibility to parasitic infection / MGI
- decreased double-negative T cell number / MGI
- increased double-negative T cell number / MGI
- decreased susceptibility to type IV hypersensitivity reaction / MGI
- abnormal stomach mucosa morphology / MGI
- abnormal intestinal epithelium morphology / MGI
- abnormal B cell number / MGI
- decreased pre-B cell number / MGI
- decreased mature B cell number / MGI
- increased immature B cell number / MGI
- decreased immature B cell number / MGI
- abnormal gamma-delta T cell differentiation / MGI
CByIco.129-Rag2tm1Fwa Cd44tm1.1Ugu/H
Status | Available to order |
EMMA ID | EM:02022 |
International strain name | CByIco.129-Rag2tm1Fwa Cd44tm1.1Ugu/H |
Alternative name | BALB/cByJIco Rag2-/- CD44v6v7-/- |
Strain type | Targeted Mutant Strains : Knock-out |
Allele/Transgene symbol | Cd44tm1.1Ugu, Rag2tm1Fwa |
Gene/Transgene symbol | Cd44, Rag2 |
Information from provider
Provider | Ursula Günthert |
Provider affiliation | Institut für Pathologie, University of Basel |
Genetic information | A loxP site was inserted 3' of exon v6 and a neomycin resistance cassette, with a 5' loxP site, was inserted 5' of exon v6 of the Cd44 gene. During insertion, 1.6 kb of sequence was lost in the exon v7 region; cre recombinase was transiently expressed in ES cells allowing the removal of neomycin cassette and exon v6. Mice lack both exon v6 and v7. Insertion of a neomycin resistance cassette into the unique exon of the Rag2 gene by homologous recombination leading to the deletion of 286 amino acids. |
Phenotypic information | Rag2-/-: No mature T and B cells. Cd44v6v7-/-: Strongly reduced symptoms in autoimmune diseases. |
References |
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Information from EMMA
Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Animals used for archiving | homozygous BALB/cByJIco, homozygous BALB/cByJIco |
Breeding at archiving centre | Maintained by intercrossing double homozygotes prior to and during archiving |
Stage of embryos | 2-cell |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Severe combined immunodeficiency due to complete RAG1/2 deficiency / Orphanet_331206
- Omenn syndrome / Orphanet_39041
- Combined immunodeficiency with granulomatosis / Orphanet_157949
MGI phenotypes (allele matching)
MGI phenotypes (gene matching)
- decreased mast cell number / MGI
- altered response to myocardial infarction / MGI
- abnormal cell morphology / MGI
- decreased mast cell histamine storage / MGI
- abnormal uterus morphology / MGI
- thin epidermis / MGI
- abnormal lipid level / MGI
- abnormal vasculogenesis / MGI
- abnormal digestive system physiology / MGI
- impaired wound healing / MGI
- impaired macrophage phagocytosis / MGI
- liver inflammation / MGI
- lung inflammation / MGI
- abnormal definitive hematopoiesis / MGI
- no abnormal phenotype detected / MGI
- abnormal CD4-positive, alpha beta T cell morphology / MGI
- abnormal neutrophil physiology / MGI
- short tibia / MGI
- impaired skin barrier function / MGI
- delayed wound healing / MGI
- decreased myocardial infarction size / MGI
- abnormal leukocyte migration / MGI
- peritoneal inflammation / MGI
- decreased susceptibility to induced arthritis / MGI
- decreased tumor growth/size / MGI
- abnormal leukocyte adhesion / MGI
- abnormal physiological neovascularization / MGI
- increased susceptibility to induced arthritis / MGI
- enhanced coordination / MGI
- decreased cholesterol level / MGI
- abnormal vascular endothelial cell physiology / MGI
- decreased susceptibility to kidney reperfusion injury / MGI
- abnormal miniature excitatory postsynaptic currents / MGI
- decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
- increased mean systemic arterial blood pressure / MGI
- abnormal CD8-positive, alpha beta T cell morphology / MGI
- abnormal response to infection / MGI
- increased susceptibility to parasitic infection / MGI
- impaired natural killer cell mediated cytotoxicity / MGI
- decreased acute inflammation / MGI
- increased acute inflammation / MGI
- decreased T cell proliferation / MGI
- digestive/alimentary phenotype / MGI
- immune system phenotype / MGI
- hematopoietic system phenotype / MGI
- abnormal CD4-positive, alpha-beta T cell physiology / MGI
- abnormal T-helper 1 physiology / MGI
- abnormal T-helper 2 physiology / MGI
- choroidal neovascularization / MGI
- decreased circulating creatinine level / MGI
- decreased blood urea nitrogen level / MGI
- abnormal vascular smooth muscle physiology / MGI
- decreased angiogenesis / MGI
- decreased susceptibility to type IV hypersensitivity reaction / MGI
- abnormal cell physiology / MGI
- abnormal vascular endothelial cell morphology / MGI
- abnormal involution of the mammary gland / MGI
- increased diameter of tibia / MGI
- abnormal osteoclast differentiation / MGI
- increased circulating tumor necrosis factor level / MGI
- decreased circulating tumor necrosis factor level / MGI
- increased tumor necrosis factor secretion / MGI
- abnormal interleukin secretion / MGI
- increased circulating interferon-gamma level / MGI
- increased circulating interleukin-2 level / MGI
- increased interleukin-1 beta secretion / MGI
- increased interleukin-10 secretion / MGI
- increased interleukin-6 secretion / MGI
- impaired neutrophil recruitment / MGI
- abnormal chemokine secretion / MGI
- decreased transforming growth factor level / MGI
- decreased sensitivity to induced cell death / MGI
- abnormal epidermal lamellar body morphology / MGI
- increased sensitivity to induced morbidity/mortality / MGI
- abnormal circulating chemokine level / MGI
- abnormal circulating cytokine level / MGI
- decreased activation-induced cell death of T cells / MGI
- increased mitochondria size / MGI
- abnormal mitochondrial crista morphology / MGI
- decreased fibroblast proliferation / MGI
- impaired leukocyte tethering or rolling / MGI
- decreased monocyte cell number / MGI
- increased granulocyte number / MGI
- decreased bone marrow cell number / MGI
- alopecia / MGI
- abnormal digestive system morphology / MGI
- abnormal intestinal epithelium morphology / MGI
- abnormal intestinal mucosa morphology / MGI
- abnormal spleen morphology / MGI
- small spleen / MGI
- spleen hypoplasia / MGI
- abnormal Peyer's patch morphology / MGI
- abnormal thymus morphology / MGI
- decreased thymocyte number / MGI
- abnormal immune system cell morphology / MGI
- decreased body size / MGI
- abnormal humoral immune response / MGI
- arrested B cell differentiation / MGI
- decreased IgG level / MGI
- decreased IgM level / MGI
- decreased IgA level / MGI
- thymus hypoplasia / MGI
- arrested T cell differentiation / MGI
- lung inflammation / MGI
- neoplasm / MGI
- abnormal B cell differentiation / MGI
- no abnormal phenotype detected / MGI
- small lymph nodes / MGI
- abnormal lymph node morphology / MGI
- abnormal lymphopoiesis / MGI
- abnormal pre-B cell morphology / MGI
- increased susceptibility to infection / MGI
- abnormal double-negative T cell morphology / MGI
- abnormal double-positive T cell morphology / MGI
- increased susceptibility to bacterial infection / MGI
- abnormal effector T cell morphology / MGI
- abnormal macrophage physiology / MGI
- abnormal B cell number / MGI
- abnormal B cell physiology / MGI
- abnormal immune system organ morphology / MGI
- colitis / MGI
- absent Peyer's patches / MGI
- increased natural killer cell mediated cytotoxicity / MGI
- erythroderma / MGI
- abnormal lymphocyte physiology / MGI
- abnormal B cell morphology / MGI
- decreased lymphocyte cell number / MGI
- decreased B cell number / MGI
- decreased T cell number / MGI
- decreased susceptibility to parasitic infection / MGI
- decreased double-negative T cell number / MGI
- increased double-negative T cell number / MGI
- decreased double-positive T cell number / MGI
- decreased T cell proliferation / MGI
- cachexia / MGI
- increased macrophage cell number / MGI
- abnormal T-helper 1 physiology / MGI
- decreased susceptibility to type IV hypersensitivity reaction / MGI
- abnormal response to transplant / MGI
- abnormal T cell morphology / MGI
- decreased NK T cell number / MGI
- decreased CD4-positive, alpha beta T cell number / MGI
- decreased CD8-positive, alpha-beta T cell number / MGI
- lymph node hypoplasia / MGI
- decreased pre-B cell number / MGI
- decreased mature B cell number / MGI
- absent mature B cells / MGI
- increased immature B cell number / MGI
- decreased immature B cell number / MGI
- abnormal gamma-delta T cell differentiation / MGI
- decreased spleen white pulp amount / MGI
- abnormal interferon secretion / MGI
- abnormal chemokine secretion / MGI
- abnormal T cell receptor V(D)J recombination / MGI
- abnormal immunoglobulin V(D)J recombination / MGI
- absent Hassall's corpuscle / MGI
- small inguinal lymph nodes / MGI
- decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
- increased DN3 thymocyte number / MGI
- abnormal stomach mucosa morphology / MGI
- small cervical lymph nodes / MGI
Literature references
- RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.;Shinkai Y, Rathbun G, Lam K P, Oltz E M, Stewart V, Mendelsohn M, Charron J, Datta M, Young F, Stall A M, ;1992;Cell;68;855-67; 1547487
- Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330
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