On this webpage you can find the EMMA strain list with further information on the strain you are interested in. When clicking on the ‘order’ button for the appropriate strain you will be guided to an online request form which you can quickly fill in and send to EMMA through the website. The request form will ask you for contact information and which type of material you would like to receive from EMMA. After receipt of your request, EMMA will contact you within two weeks.
The European Mouse Mutant Archive – EMMA strains are cryopreserved as frozen embryos and/or sperm, which are the recommended type of materials to be distributed from EMMA to the community. In case that rederivation service is needed, please consult EMMA nodes.
The available EMMA strains and the kind of available materials can be viewed using this link:
search for EMMA strains and order them
Kind of material 1
Delivery time 2
Frozen sperm 6
Rederivation from frozen stock
up to 3 heterozygous animals 7
4-6 months 8
Allele conversion service
up to 3 heterozygous animals 7
1 Requesting frozen sperm or embryos is generally recommended, wherever possible. See below for further explanation.
2 From the time when all paperwork is in place until when the material is ready for shipping at EMMA nodes.
3 In addition users have to cover all the shipping costs (including the cost for sending empty dry-shippers, where applicable).
4 In each case, risk of loss and damage pass to the user upon the EMMA node making the material available for collection by the courier agent.
5 For strains generated by use of the CRISPR technology, users also have to pay a license surcharge.
6 IVF capability is required at the recipient institution or an external service provider close-by.
7 In general, up to 3 heterozygous animals, males or females, will be shipped. Wildtype littermates could be also available upon request.
8 For special requirements longer service time may be expected.
Recommended EMMA Service Fees
The recommended EMMA service fees enable the INFRAFRONTIER partners to recover their costs and ensure that INFRAFRONTIER maintains a sustainable archiving and distribution service. In addition to these recommended EMMA service fees users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable). For strains generated by use of the CRISPR technology, users also have to pay a license surcharge. In case of cancellations of service requests, EMMA reserves the right to charge users for incurred costs. As EMMA is not a legal entity, the individual EMMA nodes will invoice users separately.
Kind of Materials and Delivery Times
Due to its methodological simplicity and reduced costs, the default archival material is frozen sperm obtained from mice that are heterozygous for the mutation(s). Please be aware that after rederivation from frozen sperm the mutant mice obtained will not be homozygous and the background may slightly differ from the original one depending on the oocytes strain used for the recovery. Frozen material and live animals of strains that are maintained on shelf can be shipped within four weeks. Should specific age, sex, or number of live animals be enquired for, it can take longer to meet the users’ requirements. Rederivation from frozen stock usually takes four to six months, if offered by an EMMA node.
EMMA strongly promotes the shipment of frozen material in an attempt to minimise the welfare issues associated with the international movement of live animals. The EMMA nodes always provide validated thawing protocols and further information about handling frozen sperm and embryos can be downloaded here. There are occasions, where EMMA would facilitate providing rederived live animals from frozen stock, e.g. when recipients are not confident to do IVF and/or embryo transfer and also don’t have specialized facilities nearby, for strains that have proven to be hard to rederive, or if the recipient country’s rules don’t allow to import frozen material. Requestors can
In general, service requests will not be processed until a fully executed MTA has been received (where applicable) and either a billing address or a purchase order number has been provided by the customer. Therefore, please note that the delivery times given above represent the time period between the date when all paperwork is in place and the date of when the material is available for shipping at EMMA nodes. The frozen material provided will be sufficient for two IVF or embryo transfer sessions. If live animals are ordered, EMMA will ship up to 3 heterozygous animals, males or females (wildtype littermates could be also available upon request).
EMMA does not generally offer breeding services to e.g. generate cohorts of homozygous mutants. However, such a service and fees may be negotiated with the respective EMMA distribution centre. EMMA screens and distributes mice/rats in accordance with FELASA protocols and guarantees that animals leaving the EMMA institutions are free of pathogens as specified by FELASA.