Strain Distribution

The European Mouse Mutant Archive – EMMA strains are cryopreserved as frozen embryos and/or sperm. Highly demanded EMMA strains are maintained on shelf to facilitate fast delivery to the users. For all other EMMA strains a rederivation service is provided.
The available EMMA strains and the kind of material in stock can be viewed using this link:
search for EMMA strains and order them

Quick Overview

Kind of material 1

Quantity

Delivery time 2

Recommended EMMA
service fee 3,4,5

Frozen embryos

40-50 embryos

4 weeks

€1600

Frozen sperm 6

2 straws/vials

4 weeks

€1600

Live animals on shelf

up to 3 heterozygous animals 7

4 weeks 8

€3200

Rederivation from frozen stock

up to 3 heterozygous animals 7

4-6 months 8

€3200

Allele conversion service
(EUCOMM/KOMP-CSD alleles)

up to 3 heterozygous animals 7

upon request

upon request

1 Requesting frozen sperm or embryos is generally recommended, wherever possible. See below for further explanation.
2 From the time when all paperwork is in place until when the material is ready for shipping at EMMA nodes.
3 In addition users have to cover all the shipping costs (including the cost for sending empty dry-shippers, where applicable).
4 In each case, risk of loss and damage pass to the user upon the EMMA node making the material available for collection by the courier agent.
5 For strains generated by use of the CRISPR technology, users also have to pay a license surcharge.
6 IVF capability is required at the recipient institution or an external service provider close-by.
7 In general, up to 3 heterozygous animals, males or females, will be shipped. Wildtype littermates could be also available upon request.
8 For special requirements longer service time may be expected.

Recommended EMMA Service Fees

The recommended EMMA service fees enable the INFRAFRONTIER partners to recover their costs and ensure that INFRAFRONTIER maintains a sustainable archiving and distribution service. In addition to these recommended EMMA service fees users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable). For strains generated by use of the CRISPR technology, users also have to pay a license surcharge. In case of cancellations of service requests, EMMA reserves the right to charge users for incurred costs. As EMMA is not a legal entity, the individual EMMA nodes will invoice users separately.

Kind of Materials and Delivery Times

Due to its methodological simplicity and reduced costs, the default archival material is frozen sperm obtained from mice that are heterozygous for the mutation(s). Please be aware that after rederivation from frozen sperm the mutant mice obtained will not be homozygous and the background may slightly differ from the original one depending on the oocytes strain used for the recovery. Frozen material and live animals of strains that are maintained on shelf can be shipped within four weeks. Should specific age, sex, or number of live animals be enquired for, it can take longer to meet the users’ requirements. Rederivation from frozen stock usually takes four to six months.

EMMA strongly promotes the shipment of frozen material in an attempt to minimise the welfare issues associated with the movement of live animals. The EMMA nodes always provide validated thawing protocols and further information about handling frozen sperm and embryos can be downloaded here. There are occasions, where EMMA would provide live animals, e.g. when recipients are not confident to do IVF and/or embryo transfer and also don’t have specialized facilities nearby, for strains that have proven to be hard to rederive, or if the recipient country’s rules don’t allow to import frozen material. Requestors can pbagnpg hf and discuss the case and concerns individually.

In general, service requests will not be processed until a fully executed MTA has been received (where applicable) and either a billing address or a purchase order number has been provided by the customer. Therefore, please note that the delivery times given above represent the time period between the date when all paperwork is in place and the date of when the material is available for shipping at EMMA nodes. The frozen material provided will be sufficient for two IVF or embryo transfer sessions. If live animals are ordered, EMMA will ship up to 3 heterozygous animals, males or females (wildtype littermates could be also available upon request).

EMMA does not generally offer breeding services to e.g. generate cohorts of homozygous mutants. However, such a service and fees may be negotiated with the respective EMMA distribution centre. EMMA screens and distributes mice/rats in accordance with FELASA protocols and guarantees that animals leaving the EMMA institutions are free of pathogens as specified by  FELASA.

Strains under Development

EMMA has newly submitted strains in various stages of preservation, development and colony establishment. These strains are not yet available for distribution or ordering. However, you are invited to register your interest in any such strains.

IKMC/IMPC Allele Conversion Service

Some mouse strains are only archived as knockout-first allele (‘tm1a allele’), but if users are interested in another allele which is not cryopreserved at EMMA, some EMMA nodes offer allele conversion as a service.

Quality Control

Read more about the standard quality control provided by INFRAFRONTIER/EMMA.

Health Monitoring Procedures

Brief description of housing system, health monitoring program and health status of the EMMA SPF live colonies.

Legal Questions

EMMA repository conditions, MTAs, policy on licensing and use restriction.

Frequently Asked Questions (FAQs)

How can I obtain mutant strains from EMMA?

On this webpage you can find the  EMMA strain list with further information on the strain you are interested in. When clicking on the ‘order’ button for the appropriate strain you will be guided to an online request form which you can quickly fill in and send to EMMA through the website. The request form will ask you for contact information and whether you wish to receive frozen sperm or embryos or live animals. After receipt of your request, EMMA will contact you within two weeks.

What can I obtain and what is the delivery time?

You can obtain sperm or embryos as frozen samples with a delivery time of four weeks. If you order live mice after rederivation EMMA will deliver within 4 to 6 months. Some strains in high demand are maintained as live colonies and can be shipped within one month. In general, service requests will not be processed until a fully executed MTA has been received (where applicable) and either a billing address or a purchase order number has been provided by the user. Therefore please note that the delivery times given above represent the time period between the date when all paperwork is in place and when the material is ready for shipping at EMMA nodes. For special requirements (e.g. certain age, sex and/or number of live mice are required), longer service time may be expected.

Whether a strain is held as frozen material or live mice on shelf is displayed in the strain details on the  EMMA strain list, under ‘Availability’. Some strains require special expertise to be reliably rederived from frozen samples. Therefore, EMMA only distributes rederived mice in these cases. As default, heterozygous material is being archived and distributed.

What does EMMA charge?

The recommended EMMA service fees for shipments of frozen material and live mice are €1600 and €3200, respectively. The fees levied by EMMA are used solely to meet the animal husbandry and stock replacement costs, and to recuperate the cost for archiving. In addition to these recommended EMMA service fees users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable). For strains generated by use of the CRISPR technology, users also have to pay a license surcharge. In case of cancellations of service requests, EMMA reserves the right to charge users for incurred costs. The individual EMMA nodes will each bill users separately.

How many embryos will I receive?

EMMA will ship two straws containing approximately 25 embryos / straw which will be sufficient to recover the stock.

What amount of spermatozoa will I receive?

The frozen material provided will be sufficient for two IVF sessions.

How many mice will I receive per strain?

If live mice are ordered, EMMA will ship up to 3 heterozygous animals, males or females (wildtype littermates could be also available upon request).

If you wish to obtain more animals, you should let us know per email after placing you request. Please do not send more than one request per strain for that purpose.

What will be the health status of the animals I receive?

EMMA screens and distributes mice in accordance with  FELASA protocols. All mice leaving the EMMA institutions are free of the pathogens specified in the FELASA guidelines.

Do I need an import license?

Yes, but the actual paperwork will vary according to your own national rules and regulations. You should seek advice from your named veterinary surgeon or animal house manager.

What do I need to know about shipment responsibilities and the role of courier agents?

The EMMA node will make the material ready for shipping. The user has to cover all the shipping costs (including the cost for sending empty dry-shippers, where applicable) and will usually task a courier agent with picking up the material from the EMMA node and delivering it safely. All material is supplied Ex Works (EXW Incoterms 2020) from the EMMA node. Risk and title in the physical material shall pass to the user upon the EMMA node making the material available for collection by the user or its agent. As to risk of loss and damage we’d like to clearly explain that in case of shipping problems, even if the material is not delivered by the courier agent, the user still has to pay the recommended EMMA service fee.

Are EMMA strains available to non-European Institutes?

EMMA strains are available to academic institutions all around the world. Stocks may also be available to industrial / commercial organisations, although their use may be restricted in accordance with specific Material Transfer Agreements held by the original owners / producers of the requested strain.

Is EMMA only distributing strains to non-profit users?

EMMA is primarily distributing strains to non-profit users. For certain collections of strains and if the provider agrees, it may be possible to distribute to commercial users as well. Companies and other for-profit entities interested in obtaining EMMA strains please Pbagnpg hf ivn R-Znvy.

Can I register interest for strains that are still under development?

EMMA has newly submitted strains in various stages of preservation, development and colony establishment. These strains are not yet available for distribution or ordering. However, you are invited to register your interest in any such strains by clicking on the  EMMA strain list, finding your strain of interest, then clicking the ‘Register interest’ button.

Registrations of interest will be handled on a first-come, first-served basis. This registration of interest is valuable in helping EMMA to anticipate demand and to optimise availability of strains. Users will be notified as soon as the strain becomes available. They will receive an e-mail with a link to a pre-filled online form that they can use to submit their order.

EMMA strains produced from ES cells generated by the International Knockout Mouse Consortium (IKMC) are displayed on the EMMA strain list as soon as germ-line transmission has been achieved and the genotype of the pups has been confirmed. From this stage on scientists can register their interest for the mice on the EMMA strain list as described above.

Genes that have only been assigned for mouse production and phenotyping by the International Mouse Phenotyping Consortium (IMPC) and for which mice have not been produced, yet, it is possible to register interest on the  IMPC webpage.

ES cells and targeting vectors can be ordered directly from the  European Mouse Mutant Cell Repository (EuMMCR) holds and distributes vectors and mutant ES cell lines produced in the EUCOMM and EUCOMMTools consortia representing over 16.800 different genes.

Can I order IKMC vectors and ES cells from EMMA?

No, IKMC vectors and ES cells must be orderd from the  IKMC repositories. The  European Mouse Mutant Cell Repository (EuMMCR) holds and distributes vectors and mutant ES cell lines produced in the EUCOMM and EUCOMMTools consortia representing over 16.800 different genes.

Does EMMA offer allele conversion for EUCOMM/KOMP lines?

So far EMMA has archived over 4700 mouse strains generated using the EUCOMM/KOMP targeting strategies. Some mouse strains are only archived as knockout-first allele (‘tm1a allele’), but if users are interested in another allele which is not cryopreserved at EMMA, some EMMA nodes offer  allele conversion as a service. Please note that this service may take longer than the delivery times for the already cryopreserved alleles and users have to pay an additional fee.

Delivery times and service fees are available upon request using the links in the availabilities section in the EMMA strain search. In case these options are note available in the EMMA strain search, please contact emma@infrafrontier.eu.

Do I need to sign an MTA when requesting an EMMA strain?

All EMMA requests are subject to the legally binding EMMA Conditions of use and all users need to accept them in the request process. In addition, if the producer of the material requires the signing of a material transfer agreement (MTA), this can be downloaded from the respective strain description page and will be provided to the recipient upon request.
Although EMMA mainly distributes to non-commercial recipients, if commercial users are interested in an EMMA strain, we will put them in contact with the strain producer and will only send the strains when an agreement is in place between the requestor and the producer.

How does the MTA execution work when requesting an EMMA strain?

When an MTA is needed for distribution of a strain, this can be downloaded from the respective strain description page and will be provided to the recipient upon request. In general, service requests will not be processed until a fully executed MTA has been received (where applicable) and either a billing address or a purchase order number has been provided by the customer. Therefore, please make sure to fill and sign the MTA and send it to the strain owner for countersignature (or to the EMMA node if countersignature not needed) as soon as possible.

How to acknowledge EUCOMM/KOMP-CSD resource provided by INFRAFRONTIER/EMMA?

We thank [insert Provider name] for providing the mutant mouse line (Allele:[state allele]), INFRAFRONTIER/EMMA (www.infrafrontier.eu, PMID: 25414328), and [insert Distributor name] from which the mouse line was distributed (EM:xxxxx[state EMMA ID]). Associated primary phenotypic information may be found at www.mousephenotype.org.

Please include in the publication the references below:

  • INFRAFRONTIER Consortium. (2015). INFRAFRONTIER–providing mutant mouse resources as research tools for the international scientific community. Nucleic Acids Res. 43, D1171–D1175.
  • Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A. (2011). A conditional knockout resource for the genome-wide study of mouse gene function. Nature. 474, 337-342.

Additional information is available on Citing this Resource.

How to acknowledge INFRAFRONTIER/EMMA?

For acknowledgement the following citation format is appropriate:

We thank [insert Provider name] for providing the mutant mouse/rat line (Allele:[state allele]), INFRAFRONTIER/EMMA (www.infrafrontier.eu, PMID: 25414328), and [insert Distributor name] from which the mouse/rat line was distributed (EM:xxxxx[state EMMA ID]).

Additional information is available on Citing this Resource.

Why does EMMA recommend to request frozen material and under which circumstances would live animals be shipped?

EMMA strongly promotes the shipment of frozen material in an attempt to minimise the welfare issues associated with the movement of live animals. The EMMA nodes always provide validated thawing protocols and further information about handling frozen sperm and embryos can be downloaded here. There are occasions, where EMMA would provide live animals, e.g.

  • When recipients are not confident to do IVF and/or embryo transfer.
  • No specialized facilities nearby.
  • For strains that have proven to be hard to rederive.
  • Recipient country’s rules don’t allow to import frozen material.

More about animal welfare in EMMA.

INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).