129S2.CD1-Tg(Myh6-Htr2b)1Lum/Orl

Status

Available to order

EMMA IDEM:05938
International strain name129S2.CD1-Tg(Myh6-Htr2b)1Lum/Orl
Alternative nameMHC-5-HT2B in 129S2
Strain typeTransgenic Strains
Allele/Transgene symbolTg(Myh6-Htr2b)1Lum
Gene/Transgene symbolTg(Myh6-Htr2b)1Lum

Information from provider

ProviderLuc Maroteaux
Provider affiliationInstitut du Fer a Moulin, INSERM UMR S-839
Genetic informationA 5.5 Kbp BamHI-KpnI restriction fragment of the mouse alpha-myosin heavy chain (MHC) was fused to a 2.0 Kbp XbaI-XbaI restriction fragment of mouse Htr2b (5-HT2BR) cDNA. The recombinant plasmid p-MHC-5-HT2B was linearized, microinjected into pronuclei of fertilized CD1 mouse eggs and implanted into pseudopregnant CD1 foster mothers.
Phenotypic informationThe MHC-5-HT2B receptor transgenic mice exhibit hypertrophic heart with increased ventricular mass, abnormal cellular organization and proliferation of mitochondria. This was not accompanied with cardiovascular functional alterations. Seven-fold overexpression of the 5-HT2B receptor in the heart leads to compensated hypertrophic cardiomyopathy associated with proliferation of the mitochondria. This observation suggests a role for mitochondria in the hypertrophic signaling that is regulated by serotonin.
Breeding historyThe original CD1 transgenic line was backcrossed more than 10 generations over the 129S2 strain and it is currently maintained over this 129S2 background.
References
  • Overexpression of the serotonin 5-HT2B receptor in heart leads to abnormal mitochondrial function and cardiac hypertrophy.;Nebigil Canan G, Jaffré Fabrice, Messaddeq Nadia, Hickel Pierre, Monassier Laurent, Launay Jean-Marie, Maroteaux Luc, ;2003;Circulation;107;3223-9; 12810613
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivingheterozygous 129S2/SvPas, wild-type 129S2/SvPas
Breeding at archiving centreSome wild-type females provided by the company potentially have a mixed background: B6.CBAx129/SvPas. It is possible that some frozen embryos have the same background.
Stage of embryos8-cell

Disease and phenotype information

MGI allele-associated human disease models

Literature references

  • Overexpression of the serotonin 5-HT2B receptor in heart leads to abnormal mitochondrial function and cardiac hypertrophy.;Nebigil Canan G, Jaffré Fabrice, Messaddeq Nadia, Hickel Pierre, Monassier Laurent, Launay Jean-Marie, Maroteaux Luc, ;2003;Circulation;107;3223-9; 12810613

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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