C57BL/6N-Lrrk1^{tm1c(KOMP)Wtsi}/H

Status	Only small colony available
EMMA ID	EM:10149
Citation information	RRID:IMSR_EM:10149 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-Lrrk1^{tm1c(KOMP)Wtsi}/H
Alternative name	EPD0073_6_C01
Strain type	Targeted Mutant Strains : Targeted Conditional
Allele/Transgene symbol	Lrrk1^{tm1c(KOMP)Wtsi}
Gene/Transgene symbol	Lrrk1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This line originates from KOMP ES clone EPD0073_6_C01, after breeding with a Flp recombinase deleter line to convert the original targeted allele tm1a (knock-out first allele) into a conditional allele tm1c. For further details on the construction of this clone see the page at the IKMC page on the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Osteosclerotic metaphyseal dysplasia / Orphanet_500548

IMPC phenotypes (gene matching)

decreased eosinophil cell number / IMPC
increased circulating phosphate level / IMPC
increased bone mineral density / IMPC
abnormal tooth morphology / IMPC
abnormal gait / IMPC
increased bone mineral content / IMPC
increased red blood cell distribution width / IMPC
abnormal vertebrae morphology / IMPC
abnormal eyelid morphology / IMPC
increased circulating cholesterol level / IMPC
abnormal fibula morphology / IMPC
abnormal locomotor behavior / IMPC
increased circulating creatine kinase level / IMPC
short tibia / IMPC
decreased lymphocyte cell number / IMPC
increased neutrophil cell number / IMPC
increased total body fat amount / IMPC
decreased lean body mass / IMPC
abnormal femur morphology / IMPC
decreased leukocyte cell number / IMPC
abnormal rib morphology / IMPC
abnormal maxilla morphology / IMPC
abnormal radius morphology / IMPC
abnormal zygomatic bone morphology / IMPC
abnormal mandible morphology / IMPC
abnormal humerus morphology / IMPC
abnormal joint morphology / IMPC
abnormal clavicle morphology / IMPC
decreased circulating alkaline phosphatase level / IMPC
increased circulating iron level / IMPC
increased circulating aspartate transaminase level / IMPC
abnormal ulna morphology / IMPC
absent teeth / IMPC
thrombocytopenia / IMPC
abnormal snout morphology / IMPC
abnormal tibia morphology / IMPC
abnormal pelvic girdle bone morphology / IMPC
increased circulating HDL cholesterol level / IMPC

MGI phenotypes (gene matching)

increased bone mineral density / MGI
abnormal bone marrow cavity morphology / MGI
osteopetrosis / MGI
abnormal trabecular bone morphology / MGI
abnormal long bone metaphysis morphology / MGI
decreased body height / MGI
abnormal osteoclast physiology / MGI
short tibia / MGI
abnormal bone mineralization / MGI
decreased circulating alkaline phosphatase level / MGI
short femur / MGI
abnormal long bone morphology / MGI
abnormal facial morphology / MGI
increased compact bone thickness / MGI
increased osteoclast cell number / MGI
decreased bone resorption / MGI
homeostasis/metabolism phenotype / MGI
craniofacial phenotype / MGI
skeleton phenotype / MGI
osteosclerosis / MGI
increased trabecular bone thickness / MGI
increased bone trabecula number / MGI
increased trabecular bone volume / MGI
preweaning lethality, incomplete penetrance / MGI
decreased susceptibility to osteoporosis / MGI

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Order (limited)

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Lrrk1tm1c(KOMP)Wtsi/H