B6.Cg-Arhgap19tm1Ins/Flmg

Status

Available to order

EMMA IDEM:10176
Citation informationRRID:IMSR_EM:10176 

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International strain nameB6.Cg-Arhgap19tm1Ins/Flmg
Alternative namearhgap19 lox/lox
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolArhgap19tm1Ins
Gene/Transgene symbolArhgap19

Information from provider

ProviderJacques Bertoglio
Provider affiliationINSERM U1170, INSTITUT GUSTAVE ROUSSY
Genetic informationLoxP sequence insertion on both sides of a 3.2 kb genomic segment containing exons 4, 5, and 6 that encode for the catalytic GAP domain of Arhgap19.
Phenotypic informationHomozygous:
No phenotype. Arhgap19 gene can be deleted by crossing to appropriate cre recombinase-expressing mice.

Heterozygous:
None. Note: we have previously characterized ARHGAP19 as a RhoGAP that is mainly expressed in hematopoietic cells and that controls mitosis in lymphocytes (David MD, Petit D, Bertoglio J. The RhoGAP ARHGAP19 controls cytokinesis and chromosome segregation in T lymphocytes. J Cell Sci. 2014 Jan 15;127:400-10. PMID:24259668). At this point we have generated Arhgap19 null mice by crossing with a PGK-cre line. Null mice are viable, with no major developmental defects. However, by the age of 6-8 months these mice develop hematopoietic abnormalities that are currently being characterized.
Breeding historySNP marker-assisted accelerated backcrossing. 100% C57BL/6 genotype was achieved at the 6th generation.
ReferencesNone available
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedyes

Information from EMMA

Archiving centreB.S.R.C. Alexander Fleming, Vari, Greece
Animals used for archivinghomozygous C57BL/6 males, wild-type C57BL/6J females
Stage of embryos2-cell

Disease and phenotype information

IMPC phenotypes (gene matching)
  • preweaning lethality, incomplete penetrance / IMPC
  • enlarged heart / IMPC
  • abnormal heart morphology / IMPC
MGI phenotypes (gene matching)
  • no abnormal phenotype detected / MGI
  • no phenotypic analysis / MGI

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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