B6.129-Shisa3tm1Ktry/Kctt

Status

Available to order

EMMA IDEM:10417
Citation informationRRID:IMSR_EM:10417 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

International strain nameB6.129-Shisa3tm1Ktry/Kctt
Alternative nameB6.129P-Shisa3 tm1Ktry
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolShisa3tm1Ktry
Gene/Transgene symbolShisa3

Information from provider

ProviderKarl Tryggvason
Provider affiliationMBB, Karolinska Institutet
Genetic informationThe genomic sequence of Shisa3 (chromosomal position: 66217811-66223359 bp on chromosome 5) from ATG to the TGA stop codon was replaced, in frame with respect to the Shisa3 initiation codon, by the coding sequence of eGFP and a loxP flanked neomycin gene driven by a mammalian promoter.
Phenotypic informationNo abnormal phenotype detected.
Breeding historyThe mice have been backcrossed more than 10 times with C57BL/6N mice. Currently they have been bred as full knockouts for 2 generations.
ReferencesNone available
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisednot known

Information from EMMA

Archiving centreKarolinska Institutet, Stockholm, Sweden
Animals used for archivinghomozygous C57BL/6N males

Disease and phenotype information

MGI phenotypes (gene matching)
  • no abnormal phenotype detected / MGI

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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