C57BL/6J-Tg(Gad2-tdTomato)DJhi/Ph
| Status | Available to order |
| EMMA ID | EM:10422 |
| Citation information | RRID:IMSR_EM:10422 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6J-Tg(Gad2-tdTomato)DJhi/Ph |
| Alternative name | B6-Tg(Gad2-tdTomato)D |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Gad2-tdTomato)DJhi |
| Gene/Transgene symbol | Tg(Gad2-tdTomato)DJhi |
Information from provider
| Provider | Johannes Hirrlinger |
| Provider affiliation | Carl-Ludwig-Institute for Physiology, University of Leipzig |
| Genetic information | The BAC clone 407K8 from the mouse C57BL/6 BAC library RPCI-23 (Research Genetics Inc., Huntsville, AL) containing the GAD2 (GAD65) gene was modified by inserting the tdTomato-ORF after the endogenous start signal. The modified BAC-GAD65-tdTomato construct was integrated into an unknown locus of C57BL/6 mice. |
| Phenotypic information | Homozygous:Transgenic mice express tdTomato in 80-90% of GABAergic, GAD65-positive cells. Fluorescent signals were observed in the olfactory bulb, striatum, cortex, hippocampus, cerebellum, brainstem and spinal cord as well as retinal ganglion and amacrine cells.Heterozygous:Should be same as for the homozygous animals. However, mouse producer does only test for presence of transgene and not for zygosity. |
| Breeding history | Transgenic founder mice were originally bred to C57BL/6JOlaHsd mice. Later mice were bred to mice from the transgenic line itself. Mice were bred for about 6 generations so far. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institute of Molecular Genetics, Prague, Czech Republic |
| Animals used for archiving | heterozygous males |
Literature references
- A Transgenic Mouse Line Expressing the Red Fluorescent Protein tdTomato in GABAergic Neurons.;Besser Stefanie, Sicker Marit, Marx Grit, Winkler Ulrike, Eulenburg Volker, Hülsmann Swen, Hirrlinger Johannes, ;2015;PloS one;10;e0129934; 26076353