B6JOla;129P2-Wnt4^tm3Svo/Oulu

Status	Available to order
EMMA ID	EM:10556
Citation information	RRID:IMSR_EM:10556 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6JOla;129P2-Wnt4^tm3Svo/Oulu
Alternative name	Wnt4mCh
Strain type	Targeted Mutant Strains : Knock-in
Allele/Transgene symbol	Wnt4^tm3Svo
Gene/Transgene symbol	Wnt4

Information from provider

Provider	Jingdong Shan
Provider affiliation	Department of Biochemistry and Molecular Medicine, University of Oulu
Genetic information	Wnt4-mCh/mCh fusion construct was targeted to the first exon of the Wnt4 locus in 129/Ola ES cell.
Phenotypic information	Homozygous: Cyst formation in the adult kidney. Mullerian duct was found to be formed in 45% of the Wnt4-mCh/mCh embryos, but in the remaining 55% it had failed to develop (HMG 2016, 25(6):1059-73). Heterozygous: Wnt4-mCherry affects slightly the heterozygous mouse
Breeding history	Targeted mutation was directly maintained on a C57BL/6 background.
References	Wnt4 coordinates directional cell migration and extension of the Müllerian duct essential for ontogenesis of the female reproductive tract.;Prunskaite-Hyyryläinen Renata, Skovorodkin Ilya, Xu Qi, Miinalainen Ilkka, Shan Jingdong, Vainio Seppo J, ;2016;Human molecular genetics;25;1059-73; 26721931
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	University of Oulu, Oulu, Finland
Animals used for archiving	heterozygous males
Stage of embryos	2-cell

Disease and phenotype information

MGI phenotypes (gene matching)

absent kidney / MGI
abnormal kidney development / MGI
delayed kidney development / MGI
decreased thymocyte number / MGI
abnormal ovary morphology / MGI
abnormal oogenesis / MGI
secondary sex reversal / MGI
no abnormal phenotype detected / MGI
abnormal secondary sex determination / MGI
abnormal adrenal gland physiology / MGI
no phenotypic analysis / MGI
kidney failure / MGI
nervous system phenotype / MGI
abnormal reproductive system development / MGI
abnormal adenohypophysis morphology / MGI
decreased renal glomerulus number / MGI
decreased oocyte number / MGI
abnormal female germ cell morphology / MGI
absent Mullerian ducts / MGI
decreased somatotroph cell number / MGI
decreased gonadotroph cell number / MGI
decreased thyrotroph cell number / MGI
oocyte degeneration / MGI
abnormal metanephric mesenchyme morphology / MGI
abnormal nephrogenic mesenchyme morphogenesis / MGI
postnatal lethality, complete penetrance / MGI
neonatal lethality, complete penetrance / MGI
small metanephros / MGI

Literature references

Wnt4 coordinates directional cell migration and extension of the Müllerian duct essential for ontogenesis of the female reproductive tract.;Prunskaite-Hyyryläinen Renata, Skovorodkin Ilya, Xu Qi, Miinalainen Ilkka, Shan Jingdong, Vainio Seppo J, ;2016;Human molecular genetics;25;1059-73; 26721931

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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