B6.Cg-Chd1tm1.1Alsr/Biat

Status

Available to order

EMMA IDEM:10600
Citation informationRRID:IMSR_EM:10600 

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International strain nameB6.Cg-Chd1tm1.1Alsr/Biat
Alternative nameChd1[delta2/delta2]
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolChd1tm1.1Alsr
Gene/Transgene symbolChd1

Information from provider

ProviderAlexandra Lusser
Provider affiliationDivision of Molecular Biology, Biocenter, Medical University of Innsbruck
Genetic informationDeletion of exon 2 of the Chd1 gene resulting in an N-terminally truncated protein.
Phenotypic informationHomozygous:
The homozygous deletion Chd1[delta2/delta2] gives rise to an N-terminally truncated protein of the chromatin remodeling and assembly factor Chd1. In general the mice appear normal and healthy and they are fertile. However, they exhibit a memory deficit (not yet published). Moreover, ex vivo culture of mutant ESCs results in compromised ESC differentiation (see Piatti et al., 2015).

Heterozygous:
Normal and healthy.
Breeding historyChimeras were crossed to albino-B6 mice. Offspring was backcrossed to C57BL/6NCrl for 3 times. Heterozygous floxed animals were intercrossed and homozygous floxed offspring was crossed to Ubi-cre deleter strain. Heterozygous Chd1[delta2/flox] were intercrossed to remove cre and to obtain homozygous Chd1[delta2/delta2]. Homozygous mice were maintained for 8 generations and then backcrossed to C57BL/6NCrl. Homozygous animals were again obtained by intercrosses for 3 generations.
References
  • Embryonic stem cell differentiation requires full length Chd1.;Piatti Paolo, Lim Chin Yan, Nat Roxana, Villunger Andreas, Geley Stephan, Shue Yan Ting, Soratroi Claudia, Moser Markus, Lusser Alexandra, ;2015;Scientific reports;5;8007; 25620209
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreUniversity of Veterinary Medicine, Vienna, Austria
Animals used for archivingheterozygous Other (please specify below) males

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

    • Intellectual disability-autism-speech apraxia-craniofacial dysmorphism syndrome / Orphanet_529965
IMPC phenotypes (gene matching)
  • abnormal eye morphology / IMPC
  • decreased mean corpuscular hemoglobin concentration / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • increased grip strength / IMPC
  • decreased red blood cell distribution width / IMPC
  • embryonic lethality prior to organogenesis / IMPC
  • abnormal embryo size / IMPC
  • anophthalmia / IMPC
  • embryonic lethality prior to tooth bud stage / IMPC
  • abnormal tooth morphology / IMPC
  • abnormal maxilla morphology / IMPC
MGI phenotypes (gene matching)
  • abnormal embryo development / MGI
  • abnormal mesoderm development / MGI
  • failure to gastrulate / MGI
  • decreased embryo size / MGI
  • abnormal developmental patterning / MGI
  • no abnormal phenotype detected / MGI
  • absent extraembryonic ectoderm / MGI
  • no phenotypic analysis / MGI
  • abnormal cell cycle / MGI
  • abnormal rostral-caudal axis patterning / MGI
  • embryonic lethality, complete penetrance / MGI
  • small embryonic epiblast / MGI
  • increased embryonic epiblast cell apoptosis / MGI
  • absent anterior visceral endoderm / MGI

Literature references

  • Embryonic stem cell differentiation requires full length Chd1.;Piatti Paolo, Lim Chin Yan, Nat Roxana, Villunger Andreas, Geley Stephan, Shue Yan Ting, Soratroi Claudia, Moser Markus, Lusser Alexandra, ;2015;Scientific reports;5;8007; 25620209

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Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

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Practical information

Example health report
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Material Transfer Agreement (MTA)
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