B6N.129(Cg)-Npy1rtm1.1Ceva/Kctt

Status

Available to order

EMMA IDEM:10662
Citation informationRRID:IMSR_EM:10662 

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International strain nameB6N.129(Cg)-Npy1rtm1.1Ceva/Kctt
Alternative nameNpy1rtm1.1Ceva
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolNpy1rtm1.1Ceva
Gene/Transgene symbolNpy1r

Information from provider

ProviderCarola Eva
Provider affiliationDepartment of Neuroscience, ICO, Neuroscience Institute of the Cavalieri Ottolenghi Foundation
Genetic informationThe gene targeted modified Npy1r allele in embryonic stem cells was generated by inserting one loxP site together with a neo marker flanked by two FLP recognition target sites (frt) before the ATG start codon and another loxP site after the stop codon of the Npy1r gene. After homologous recombination one correct ES cell clone was used to generate a stable heterozygous Npy1r+/loxPneo mouse line. The Neo gene was subsequently removed by FLP recombinase-mediated recombination in vivo, by crossing Npy1r+/loxPneo offsprings with FLP-expressing deleter mice (Dymecki SM, 1996: Flp recombinase promotes site-specie DNA recombination in embryonic stem cells and transgenic mice. PNAS USA 93:6191e6196) to create mouse line Npyr1tm1Ceva (lab name y12f-neo).
Phenotypic informationHomozygous:
Homozygous mice are viable and both females and males can be used for mating.

Heterozygous:
Heterozygous mice are viable and both females and males can be used for mating.
Breeding historyIn 2011 mating of C57BL/6N females and heterozygous Npyr1tm1Ceva male mice produced 358 cryo-conserved embryos for EMMA.
References
  • Regulatory functions of limbic Y1 receptors in body weight and anxiety uncovered by conditional knockout and maternal care.;Bertocchi Ilaria, Oberto Alessandra, Longo Angela, Mele Paolo, Sabetta Marianna, Bartolomucci Alessandro, Palanza Paola, Sprengel Rolf, Eva Carola, ;2011;Proceedings of the National Academy of Sciences of the United States of America;108;19395-400; 22084082
  • Conditional inactivation of neuropeptide Y Y1 receptors unravels the role of Y1 and Y5 receptors coexpressing neurons in anxiety.;Longo Angela, Mele Paolo, Bertocchi Ilaria, Oberto Alessandra, Bachmann Alessia, Bartolomucci Alessandro, Palanza Paola, Sprengel Rolf, Eva Carola, ;2014;Biological psychiatry;76;840-9; 24548641
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreKarolinska Institutet, Stockholm, Sweden
Animals used for archivingheterozygous C57BL/6N males, wild-type C57BL/6N females
Stage of embryos2-cell

Disease and phenotype information

MGI phenotypes (gene matching)
  • abnormal vasoconstriction / MGI
  • abnormal spleen morphology / MGI
  • small spleen / MGI
  • abnormal sympathetic system morphology / MGI
  • increased body weight / MGI
  • obese / MGI
  • increased aggression towards mice / MGI
  • hyperactivity / MGI
  • hypoactivity / MGI
  • decreased exploration in new environment / MGI
  • abnormal eating behavior / MGI
  • abnormal cardiovascular system physiology / MGI
  • increased circulating free fatty acid level / MGI
  • hyperglycemia / MGI
  • increased circulating corticosterone level / MGI
  • abnormal immune system physiology / MGI
  • abnormal immune cell physiology / MGI
  • abnormal T cell activation / MGI
  • abnormal inflammatory response / MGI
  • increased litter size / MGI
  • abnormal glucose homeostasis / MGI
  • increased circulating insulin level / MGI
  • no abnormal phenotype detected / MGI
  • abnormal macrophage physiology / MGI
  • abnormal professional antigen presenting cell physiology / MGI
  • increased immunoglobulin level / MGI
  • abnormal immunoglobulin level / MGI
  • increased IgM level / MGI
  • increased IgA level / MGI
  • abnormal immune system organ morphology / MGI
  • altered righting response / MGI
  • no phenotypic analysis / MGI
  • increased alcohol consumption / MGI
  • abnormal postnatal subventricular zone morphology / MGI
  • abnormal rostral migratory stream morphology / MGI
  • decreased energy expenditure / MGI
  • decreased neuronal precursor cell number / MGI
  • decreased B cell number / MGI
  • decreased oxygen consumption / MGI
  • impaired glucose tolerance / MGI
  • decreased heart rate / MGI
  • behavior/neurological phenotype / MGI
  • abnormal glycogen level / MGI
  • abnormal energy expenditure / MGI
  • increased percent body fat/body weight / MGI
  • abnormal T-helper 1 physiology / MGI
  • cardiac interstitial fibrosis / MGI
  • decreased susceptibility to type IV hypersensitivity reaction / MGI
  • increased circulating leptin level / MGI
  • abnormal olfactory sensory neuron morphology / MGI
  • abnormal T cell number / MGI
  • increased CD4-positive, alpha beta T cell number / MGI
  • increased CD8-positive, alpha-beta T cell number / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • increased single-positive T cell number / MGI
  • decreased marginal zone B cell number / MGI
  • decreased transitional stage B cell number / MGI
  • decreased mature B cell number / MGI
  • decreased IgG2a level / MGI
  • increased IgG2b level / MGI
  • decreased circulating tumor necrosis factor level / MGI
  • decreased interferon-gamma secretion / MGI
  • decreased circulating interleukin-12 level / MGI
  • abnormal splenic cell ratio / MGI
  • abnormal lymph node cell ratio / MGI
  • increased gonadal fat pad weight / MGI
  • increased epididymal fat pad weight / MGI
  • increased retroperitoneal fat pad weight / MGI
  • impaired behavioral response to alcohol / MGI
  • decreased sensitivity to xenobiotic induced morbidity/mortality / MGI
  • abnormal neuron differentiation / MGI
  • increased total body fat amount / MGI
  • mortality/aging / MGI
  • decreased food intake / MGI
  • decreased compensatory feeding amount / MGI
  • increased adipocyte glucose uptake / MGI

Literature references

  • Regulatory functions of limbic Y1 receptors in body weight and anxiety uncovered by conditional knockout and maternal care.;Bertocchi Ilaria, Oberto Alessandra, Longo Angela, Mele Paolo, Sabetta Marianna, Bartolomucci Alessandro, Palanza Paola, Sprengel Rolf, Eva Carola, ;2011;Proceedings of the National Academy of Sciences of the United States of America;108;19395-400; 22084082
  • Conditional inactivation of neuropeptide Y Y1 receptors unravels the role of Y1 and Y5 receptors coexpressing neurons in anxiety.;Longo Angela, Mele Paolo, Bertocchi Ilaria, Oberto Alessandra, Bachmann Alessia, Bartolomucci Alessandro, Palanza Paola, Sprengel Rolf, Eva Carola, ;2014;Biological psychiatry;76;840-9; 24548641

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

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* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
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EMMA conditions
Legally binding conditions for the transfer

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