C57BL/6-Tg(Gt(ROSA)26Sor-ALPP)181Biat/Biat
| Status | Available to order |
| EMMA ID | EM:10665 |
| Citation information | RRID:IMSR_EM:10665 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6-Tg(Gt(ROSA)26Sor-ALPP)181Biat/Biat |
| Alternative name | C57BL/6N-Tg(R26-ALPP)181Biat [Tg(ALPP)] LabName, Synonyms: ALPP donor strain (ALPP181), Tg(ALPP), li |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Gt(ROSA)26Sor-ALPP)181Biat |
| Gene/Transgene symbol | Tg(Gt(ROSA)26Sor-ALPP)181Biat |
Information from provider
| Provider | Reinhold Erben |
| Provider affiliation | Department 1 (Biomedical Sciences), University of Veterinary Medicine |
| Genetic information | This animal model is based on a dual transgenic system, consisting of a donor line (line 1) and a corresponding marker-tolerant recipient line (line 2) characterized by innate tolerance to the donor marker. ALPP (human placental alkaline phosphatase) was chosen as cell-tracking marker protein due to its ease of detection in several tissues, and because ALPP is known to be developmentally neutral in mice. The donor line Tg(ALPP) expresses heat stable wild-type ALPP in a ubiquitous fashion and serves as an unlimited source of genetically labeled cells and tissues. |
| Phenotypic information | Homozygous:No overt phenotype; histology: ubiquitously positive staining (heat stable) of the human PLAP protein in transgenic animals.Heterozygous:No overt phenotype; histology: ubiquitously positive staining (heat stable) of the human PLAP protein in transgenic animals. |
| Breeding history | Created in 2007 in C57BL/6, constantly crossed with C57BL/6N (20 generations minimum); breeding system: tg x C57BL/6; next generation: C57BL/6 x tg (male and female heredity alternating); keeping line 1 and 2 on an identical background is essential for this animal model ! Background differences may cause immunoreactions in transplantation experiments independently from ALPP. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
| Animals used for archiving | heterozygous C57BL/6N males |
Literature references
- Tracking mesenchymal stem cell contributions to regeneration in an immunocompetent cartilage regeneration model.;Zwolanek Daniela, Satué María, Proell Verena, Godoy José R, Odörfer Kathrin I, Flicker Magdalena, Hoffmann Sigrid C, Rülicke Thomas, Erben Reinhold G, ;2017;JCI insight;2;; 29046476