C57BL/6-Tg(Gt(ROSA)26Sor-ALPP*)192Biat/Biat
| Status | Available to order |
| EMMA ID | EM:10666 |
| Citation information | RRID:IMSR_EM:10666 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6-Tg(Gt(ROSA)26Sor-ALPP*)192Biat/Biat |
| Alternative name | C57BL/6N-Tg(R26-ALPPm)192Biat [Tg(ALPPm)] ; LabNames: TG(ALPP |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Gt(ROSA)26Sor-ALPP*)192Biat |
| Gene/Transgene symbol | Tg(Gt(ROSA)26Sor-ALPP*)192Biat |
Information from provider
| Provider | Reinhold Erben |
| Provider affiliation | Department 1 (Biomedical Sciences), University of Veterinary Medicine, Vienna |
| Genetic information | To generate ALPP (human placental alkaline phosphatase) tolerant recipients (line 2), ALPP's unique heat stability was used: changing glutamic acid 451 to glycine was sufficient to render the protein heat-labile. This heat-labile form of ALPP can easily be distinguished from wild-type ALPP by heat inactivation, similar to endogenous APs. These recipient animals show complete immuno-tolerance versus wild-type ALPP (versus cells/tissues transplanted from the donor line), as this mutated form of ALPP is virtually identical to native ALPP. Mutations: human alkaline phosphatase (hPLAP) and Gt(ROSA)26Sor promoter (R26) was amplified from transgenic Fischer 344 rats ubiquitously expressing hPLAP (Kisseberth et al., 1999, Developmental Biology 214, 128-138). A single point mutation (A to G) was introduced via splice overlap extension resulting in an amino acid substitution at position 429 (glutamic acid to glycine). Effect: loss of heat stability of the gene product. Transgenic animals were created by pronuclear injection of mouse zygotes and random integration of the transgene. Phenotype: ubiquitous expression (low level) of the mutated marker protein hPLAP (heat labile); size of transgene: 5142 bp. |
| Phenotypic information | Homozygous:No overt phenotype; histology: ubiquitously positive staining of the human PLAP protein in transgenic animals, gone after heat inactivation (like mouse AP).Heterozygous:No overt phenotype; histology: ubiquitously positive staining of the human PLAP protein in transgenic animals, gone after heat inactivation (like mouse AP). |
| Breeding history | Created in 2007 on C57BL/6, constantly crossed with C57BL/6N (20 generations minimum); breeding system: tg x C57BL/6; next generation: C57BL/6 x tg (male and female heredity alternating) |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
| Animals used for archiving | heterozygous C57BL/6N males |
Literature references
- Tracking mesenchymal stem cell contributions to regeneration in an immunocompetent cartilage regeneration model.;Zwolanek Daniela, Satué María, Proell Verena, Godoy José R, Odörfer Kathrin I, Flicker Magdalena, Hoffmann Sigrid C, Rülicke Thomas, Erben Reinhold G, ;2017;JCI insight;2;; 29046476