B6;129S7-Lhcgr^tm1Hht/Oulu

Status	Available to order
EMMA ID	EM:10681
Citation information	RRID:IMSR_EM:10681 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6;129S7-Lhcgr^tm1Hht/Oulu
Alternative name	Lhcgrtm1Hht, LuRKO mice
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Lhcgr^tm1Hht
Gene/Transgene symbol	Lhcgr

Information from provider

Provider	Ilpo Huhtaniemi
Provider affiliation	Biomedicine/Physiology, University of Turku
Genetic information	Lhcgr gene exon 11, which encodes the transmembrane and cytoplasmic receptor domains, was replaced with a neomycin selection gene by homologous recombination.
Phenotypic information	Homozygous: The mice are born healthy with normal male and female appearance. However, in both sexes the LHR inactivation leads to multiple alterations in the reproductive tract starting at puberty. The lack of gonadal negative feedback on the hypothalamic-pituitary level induces elevated LH and FSH levels, whereas due to the gonadal unresponsiveness to LH circulating sex steroid concentrations are decreased, resulting in infertility in both sexes. In females the final preovulatory maturation of follicles is blocked, and consequently the females do not ovulate. The Graafian follicles do not develop beyond the antral stage and the ovaries reveal increased follicular apoptosis. In males, spermatogenesis is arrested at the round spermatid stage, and the number and size of Leydig cells are remarkably reduced. Heterozygous: Heterozygous LuRKO mice are fertile.
References	Normal prenatal but arrested postnatal sexual development of luteinizing hormone receptor knockout (LuRKO) mice.;Zhang F P, Poutanen M, Wilbertz J, Huhtaniemi I, ;2001;Molecular endocrinology (Baltimore, Md.);15;172-83; 11145748 Testosterone replacement therapy induces spermatogenesis and partially restores fertility in luteinizing hormone receptor knockout mice.;Pakarainen Tomi, Zhang Fu-Ping, Mäkelä Sari, Poutanen Matti, Huhtaniemi Ilpo, ;2005;Endocrinology;146;596-606; 15514086 Knockout of luteinizing hormone receptor abolishes the effects of follicle-stimulating hormone on preovulatory maturation and ovulation of mouse graafian follicles.;Pakarainen Tomi, Zhang Fu-Ping, Nurmi Laura, Poutanen Matti, Huhtaniemi Ilpo, ;2005;Molecular endocrinology (Baltimore, Md.);19;2591-602; 15941853 Fertility in luteinizing hormone receptor-knockout mice after wild-type ovary transplantation demonstrates redundancy of extragonadal luteinizing hormone action.;Pakarainen Tomi, Zhang Fu-Ping, Poutanen Matti, Huhtaniemi Ilpo, ;2005;The Journal of clinical investigation;115;1862-8; 15951841
Homozygous fertile	no
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	University of Oulu, Oulu, Finland
Animals used for archiving	heterozygous males

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Leydig cell hypoplasia due to partial LH resistance / Orphanet_96266
Leydig cell hypoplasia due to complete LH resistance / Orphanet_96265
Familial male-limited precocious puberty / Orphanet_3000

IMPC phenotypes (gene matching)

small stomach / IMPC
decreased brain size / IMPC
small liver / IMPC
small lung / IMPC
small thymus / IMPC
increased lymphocyte cell number / IMPC
small spleen / IMPC
small heart / IMPC
decreased mean corpuscular volume / IMPC
increased leukocyte cell number / IMPC
thrombocytopenia / IMPC

MGI phenotypes (allele matching)

enlarged adrenal glands / MGI
small prostate gland anterior lobe / MGI
bulbourethral gland hypoplasia / MGI
enlarged spleen / MGI
abnormal female reproductive system morphology / MGI
abnormal uterus morphology / MGI
small ovary / MGI
impaired ovarian folliculogenesis / MGI
absent corpus luteum / MGI
abnormal male reproductive system morphology / MGI
small testis / MGI
small seminiferous tubules / MGI
arrest of spermatogenesis / MGI
small seminal vesicle / MGI
abnormal prostate gland morphology / MGI
decreased body weight / MGI
abnormal ejaculation / MGI
increased circulating follicle stimulating hormone level / MGI
increased circulating luteinizing hormone level / MGI
abnormal reproductive system physiology / MGI
reduced male fertility / MGI
male infertility / MGI
female infertility / MGI
reproductive system inflammation / MGI
cryptorchism / MGI
abnormal sexual interaction / MGI
delayed vaginal opening / MGI
abnormal caput epididymis morphology / MGI
oligozoospermia / MGI
decreased circulating testosterone level / MGI
abnormal testes secretion / MGI
abnormal ovarian secretion / MGI
absent Leydig cells / MGI
epididymal inflammation / MGI
prostate gland inflammation / MGI
scrotum hypoplasia / MGI
increased kidney weight / MGI
abnormal sperm physiology / MGI
decreased testis weight / MGI
epididymis hypoplasia / MGI
prostate gland hypoplasia / MGI
seminal vesicle hypoplasia / MGI
small penis / MGI
abnormal anogenital distance / MGI
Leydig cell hypoplasia / MGI
absent estrous cycle / MGI
absent estrus / MGI
thin uterus / MGI
thin uterine horn / MGI
absent endometrial glands / MGI
external male genitalia atrophy / MGI
vas deferens hypoplasia / MGI
increased adrenal gland weight / MGI

MGI phenotypes (gene matching)

abnormal abdominal fat pad morphology / MGI
enlarged adrenal glands / MGI
small prostate gland anterior lobe / MGI
bulbourethral gland hypoplasia / MGI
enlarged spleen / MGI
abnormal female reproductive system morphology / MGI
abnormal uterus morphology / MGI
abnormal ovary morphology / MGI
small ovary / MGI
impaired ovarian folliculogenesis / MGI
absent mature ovarian follicles / MGI
absent corpus luteum / MGI
abnormal vagina orifice morphology / MGI
abnormal male reproductive system morphology / MGI
small testis / MGI
Leydig cell hyperplasia / MGI
small seminiferous tubules / MGI
arrest of spermatogenesis / MGI
small seminal vesicle / MGI
abnormal prostate gland morphology / MGI
increased body weight / MGI
decreased body weight / MGI
abnormal ejaculation / MGI
increased circulating follicle stimulating hormone level / MGI
increased circulating luteinizing hormone level / MGI
abnormal reproductive system physiology / MGI
reduced male fertility / MGI
male infertility / MGI
female infertility / MGI
testis hypoplasia / MGI
abnormal seminiferous tubule morphology / MGI
reproductive system inflammation / MGI
cryptorchism / MGI
abnormal sexual interaction / MGI
abnormal epididymis morphology / MGI
delayed vaginal opening / MGI
abnormal caput epididymis morphology / MGI
abnormal cauda epididymis morphology / MGI
oligozoospermia / MGI
decreased circulating luteinizing hormone level / MGI
small prostate gland / MGI
decreased circulating testosterone level / MGI
increased circulating testosterone level / MGI
abnormal testes secretion / MGI
abnormal ovarian secretion / MGI
absent Leydig cells / MGI
decreased circulating follicle stimulating hormone level / MGI
enlarged seminal vesicle / MGI
early sexual maturation / MGI
abnormal vagina development / MGI
epididymal inflammation / MGI
prostate gland inflammation / MGI
scrotum hypoplasia / MGI
increased kidney weight / MGI
abnormal sperm physiology / MGI
decreased testis weight / MGI
decreased ovary weight / MGI
increased seminal vesicle weight / MGI
decreased seminal vesicle weight / MGI
increased epididymis weight / MGI
decreased epididymis weight / MGI
small epididymis / MGI
epididymis hypoplasia / MGI
abnormal epididymis epithelium morphology / MGI
enlarged prostate gland / MGI
increased prostate gland weight / MGI
prostate gland hypoplasia / MGI
seminal vesicle hypoplasia / MGI
decreased circulating estradiol level / MGI
increased circulating estradiol level / MGI
decreased circulating progesterone level / MGI
small penis / MGI
abnormal anogenital distance / MGI
reproductive system phenotype / MGI
Leydig cell hypoplasia / MGI
thin myometrium / MGI
thin endometrium / MGI
abnormal male germ cell apoptosis / MGI
absent estrous cycle / MGI
absent estrus / MGI
thin uterus / MGI
thin uterine horn / MGI
decreased endometrial gland number / MGI
absent endometrial glands / MGI
external male genitalia atrophy / MGI
vas deferens hypoplasia / MGI
abnormal testosterone level / MGI
increased urine major urinary protein level / MGI
increased adrenal gland weight / MGI
abnormal adult Leydig cell differentiation / MGI
decreased epididymal cell proliferation / MGI

Literature references

Normal prenatal but arrested postnatal sexual development of luteinizing hormone receptor knockout (LuRKO) mice.;Zhang F P, Poutanen M, Wilbertz J, Huhtaniemi I, ;2001;Molecular endocrinology (Baltimore, Md.);15;172-83; 11145748
Testosterone replacement therapy induces spermatogenesis and partially restores fertility in luteinizing hormone receptor knockout mice.;Pakarainen Tomi, Zhang Fu-Ping, Mäkelä Sari, Poutanen Matti, Huhtaniemi Ilpo, ;2005;Endocrinology;146;596-606; 15514086
Knockout of luteinizing hormone receptor abolishes the effects of follicle-stimulating hormone on preovulatory maturation and ovulation of mouse graafian follicles.;Pakarainen Tomi, Zhang Fu-Ping, Nurmi Laura, Poutanen Matti, Huhtaniemi Ilpo, ;2005;Molecular endocrinology (Baltimore, Md.);19;2591-602; 15941853
Fertility in luteinizing hormone receptor-knockout mice after wild-type ovary transplantation demonstrates redundancy of extragonadal luteinizing hormone action.;Pakarainen Tomi, Zhang Fu-Ping, Poutanen Matti, Huhtaniemi Ilpo, ;2005;The Journal of clinical investigation;115;1862-8; 15951841

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6;129S7-Lhcgrtm1Hht/Oulu