B6.Cg-Tg(Thy1-SERPINI1*S49P)680Zbz/Biat
| Status | Available to order |
| EMMA ID | EM:10871 |
| Citation information | RRID:IMSR_EM:10871 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Tg(Thy1-SERPINI1*S49P)680Zbz/Biat |
| Alternative name | Tg(NS-S49P)680Zbz |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Thy1-SERPINI1*S49P)680Zbz |
| Gene/Transgene symbol | Tg(Thy1-SERPINI1*S49P)680Zbz |
Information from provider
| Provider | Thomas Ruelicke |
| Provider affiliation | Department 1 (Biomedical Sciences), University of Veterinary Medicine, Vienna |
| Genetic information | Transgenic mice overexpressing human neuroserpin with the S49P-Syracuse mutation under the control of the Thy1 promoter were generated by pronuclear injection into fertilized oocytes using an expression cassette derived from the murine Thy1 gene. The S49P-Syracuse mutation was introduced into the human neuroserpin cDNA using the polymerase chain reaction technique. The transgene was expressed in central nervous system neurons throughout postnatal life. The expression levels for transgenic mice overexpressing human Syracuse neuroserpin were for transgenic mice with human wild-type neuroserpin 3.4 times above the endogenous neuroserpin level of wild-type mice. |
| Phenotypic information | Homozygous:unknownHeterozygous:See attached paper "Accumulation of Mutant Neuroserpin Precedes Development of Clinical Symptoms in Familial Encephalopathy with Neuroserpin Inclusion Bodies" |
| Breeding history | The transgenic lines were generated on a B6D2F2 hybrid background and backcrossed to C57BL/6J for at least 10 generations. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
| Animals used for archiving | heterozygous C57BL/6J males |
Literature references
- Accumulation of mutant neuroserpin precedes development of clinical symptoms in familial encephalopathy with neuroserpin inclusion bodies.;Galliciotti Giovanna, Glatzel Markus, Kinter Jochen, Kozlov Serguei V, Cinelli Paolo, Rülicke Thomas, Sonderegger Peter, ;2007;The American journal of pathology;170;1305-13; 17392169