B6.Cg-Cyp1a1^tm1.1Dwn Cyp1a2^tm1Dwn Cyp1b1^tm1Gonz/H

Status	Available to order
EMMA ID	EM:11097
Citation information	RRID:IMSR_EM:11097 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.Cg-Cyp1a1^tm1.1Dwn Cyp1a2^tm1Dwn Cyp1b1^tm1Gonz/H
Alternative name	Triple Cyp
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Cyp1a1^tm1.1Dwn, Cyp1a2^tm1Dwn, Cyp1b1^tm1Gonz
Gene/Transgene symbol	Cyp1a1, Cyp1a2, Cyp1b1

Information from provider

Provider	Brigitta Stockinger
Provider affiliation	Mill Hill Laboratory, The Francis Crick Institute
Genetic information	Stock that is homozygous for three knock-outs: Cyp1a1^tm1.1Dwn, Cyp1a2^tm1Dwn and Cyp1b1^tm1Gonz. Stock described as being fully backcrossed to C57BL/6 (initially with C57BL/6J, but latterly with two generations of backcrossing to C57BL/6Nimr).
Phenotypic information	Homozygous: Mice homozygous for all three knock-out alleles at Cyp1a1, Cyp1a2 and Cyp1b1 have an increased risk of death before embryonic day 11, and exhibit (with reduced penetrance) hydrocephalus, hermaphroditism and cystic ovaries. They also show slower weight gain, but are also prone to develop spontaneous obesity. The activity of many genes has been shown to be perturbed including those involved in: lipid, steroid and cholesterol biosynthesis and metabolism; metal-ion binding; nucleosome and chromatin assembly (see Dragin et al., 2008, Mol Pharmacol 73, (6), 1844-56). Heterozygous: Not known; probably no overt phenotype.
Breeding history	Stock described as being fully backcrossed to C57BL/6 (initially with C57BL/6J, but latterly with two generations of backcrossing to C57BL/6Nimr). Strain has been bred to homozygosity.
References	Feedback control of AHR signalling regulates intestinal immunity.;Schiering Chris, Wincent Emma, Metidji Amina, Iseppon Andrea, Li Ying, Potocnik Alexandre J, Omenetti Sara, Henderson Colin J, Wolf C Roland, Nebert Daniel W, Stockinger Brigitta, ;2017;Nature;542;242-245; 28146477
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	homozygous C57BL/6 males
Breeding at archiving centre	C57BL/6 background

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Peters anomaly / Orphanet_708
Congenital glaucoma / Orphanet_98976

MGI phenotypes (allele matching)

decreased hematocrit / MGI
increased neutrophil cell number / MGI
abnormal liver physiology / MGI
increased circulating alanine transaminase level / MGI
increased liver weight / MGI
decreased spleen weight / MGI
decreased thymus weight / MGI
decreased lymphocyte cell number / MGI
increased circulating aspartate transaminase level / MGI
abnormal hemoglobin content / MGI
increased hemoglobin content / MGI
increased physiological sensitivity to xenobiotic / MGI
decreased physiological sensitivity to xenobiotic / MGI
abnormal xenobiotic pharmacokinetics / MGI
decreased sensitivity to xenobiotic induced morbidity/mortality / MGI
increased susceptibility to weight loss / MGI
decreased leukocyte cell number / MGI
enlarged liver / MGI
small spleen / MGI
small thymus / MGI
abnormal iridocorneal angle / MGI
hypoplastic trabecular meshwork / MGI
abnormal canal of Schlemm morphology / MGI
decreased incidence of tumors by chemical induction / MGI
absent Schlemm's canal / MGI
abnormal trabecular meshwork morphology / MGI
anterior iris synechia / MGI

MGI phenotypes (gene matching)

decreased hematocrit / MGI
increased neutrophil cell number / MGI
decreased leukocyte cell number / MGI
enlarged liver / MGI
abnormal liver physiology / MGI
small spleen / MGI
small thymus / MGI
no abnormal phenotype detected / MGI
increased circulating alanine transaminase level / MGI
increased liver weight / MGI
no phenotypic analysis / MGI
decreased spleen weight / MGI
decreased thymus weight / MGI
decreased lymphocyte cell number / MGI
increased circulating aspartate transaminase level / MGI
homeostasis/metabolism phenotype / MGI
cardiovascular system phenotype / MGI
abnormal hemoglobin content / MGI
increased hemoglobin content / MGI
abnormal physiological response to xenobiotic / MGI
increased physiological sensitivity to xenobiotic / MGI
decreased physiological sensitivity to xenobiotic / MGI
abnormal xenobiotic pharmacokinetics / MGI
decreased sensitivity to xenobiotic induced morbidity/mortality / MGI
increased susceptibility to weight loss / MGI
abnormal liver physiology / MGI
abnormal lung development / MGI
atelectasis / MGI
cyanosis / MGI
respiratory distress / MGI
postnatal lethality / MGI
abnormal pulmonary alveolar duct morphology / MGI
abnormal type II pneumocyte morphology / MGI
no phenotypic analysis / MGI
decreased incidence of tumors by chemical induction / MGI
abnormal surfactant secretion / MGI
liver/biliary system phenotype / MGI
endocrine/exocrine gland phenotype / MGI
cardiovascular system phenotype / MGI
decreased body temperature / MGI
abnormal physiological response to xenobiotic / MGI
decreased physiological sensitivity to xenobiotic / MGI
abnormal xenobiotic pharmacokinetics / MGI
absent gastric milk in neonates / MGI
enhanced behavioral response to xenobiotic / MGI
neonatal lethality, incomplete penetrance / MGI
abnormal iridocorneal angle / MGI
hypoplastic trabecular meshwork / MGI
absent Schlemm's canal / MGI
decreased incidence of tumors by chemical induction / MGI
abnormal trabecular meshwork morphology / MGI
abnormal canal of Schlemm morphology / MGI
decreased physiological sensitivity to xenobiotic / MGI
anterior iris synechia / MGI

Literature references

Feedback control of AHR signalling regulates intestinal immunity.;Schiering Chris, Wincent Emma, Metidji Amina, Iseppon Andrea, Li Ying, Potocnik Alexandre J, Omenetti Sara, Henderson Colin J, Wolf C Roland, Nebert Daniel W, Stockinger Brigitta, ;2017;Nature;542;242-245; 28146477

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B6.Cg-Cyp1a1tm1.1Dwn Cyp1a2tm1Dwn Cyp1b1tm1Gonz/H