IMPC phenotypes (gene matching)
B6.129P2-Ccr2tm1.1Blck Ccr5tm3.1Blck/Biat
| Status | Available to order |
| EMMA ID | EM:11166 |
| Citation information | RRID:IMSR_EM:11166 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129P2-Ccr2tm1.1Blck Ccr5tm3.1Blck/Biat |
| Alternative name | 25-Bd |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Ccr2tm1.1Blck, Ccr5tm3.1Blck |
| Gene/Transgene symbol | Ccr2, Ccr5 |
Information from provider
| Provider | Bruno Luckow |
| Provider affiliation | Nephrologisches Zentrum, Arbeitsgruppe Klinische Biochemie, Klinikum der Universitaet Muenchen, Medizinische Klinik und Poliklinik IV |
| Genetic information | The chemokine receptors Ccr2 and Ccr5 have been inactivated by two rounds of homologous recombination in ES cells to generate the Ccr2/Ccr5 double knock-out mice. The Ccr2/Ccr5 double-deficient mice have been backcrossed to the C57BL/6NCrl genetic background. Contain autofluorescent EGFP and DsRed2 reporter genes at the inactivated Ccr2 and Ccr5 loci. The floxed neomycin and hygromycin selection cassettes have been deleted by cre-mediated recombination. |
| Phenotypic information | Homozygous:Homozygous animals appear healthy and fertile and show unchallenged no obvious phenotype. So far not tested in any disease model.Heterozygous:Unknown, but most likely wildtype phenotype |
| Breeding history | N15+F4 |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
| Animals used for archiving | heterozygous Other (please specify below) males |
Disease and phenotype information
MGI phenotypes (gene matching)
- abnormal trabecular bone morphology / MGI
- decreased neutrophil cell number / MGI
- decreased monocyte cell number / MGI
- altered response to myocardial infarction / MGI
- enlarged liver / MGI
- abnormal spleen morphology / MGI
- enlarged spleen / MGI
- weight loss / MGI
- retinal degeneration / MGI
- abnormal osteoclast physiology / MGI
- hepatic necrosis / MGI
- multifocal hepatic necrosis / MGI
- liver inflammation / MGI
- decreased inflammatory response / MGI
- abnormal glucose homeostasis / MGI
- abnormal lipid homeostasis / MGI
- decreased airway responsiveness / MGI
- increased susceptibility to bacterial infection / MGI
- increased susceptibility to viral infection / MGI
- abnormal leukocyte physiology / MGI
- abnormal macrophage morphology / MGI
- increased IgG level / MGI
- alcohol aversion / MGI
- abnormal monocyte morphology / MGI
- abnormal immune serum protein physiology / MGI
- abnormal conditioned taste aversion behavior / MGI
- increased circulating alanine transaminase level / MGI
- abnormal cytokine secretion / MGI
- no phenotypic analysis / MGI
- abnormal leukocyte migration / MGI
- decreased tumor growth/size / MGI
- increased alcohol consumption / MGI
- decreased alcohol consumption / MGI
- abnormal leukocyte adhesion / MGI
- abnormal microglial cell physiology / MGI
- impaired macrophage chemotaxis / MGI
- decreased macrophage cell number / MGI
- increased hepatocyte apoptosis / MGI
- liver hemorrhage / MGI
- decreased susceptibility to kidney reperfusion injury / MGI
- decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
- abnormal vascular wound healing / MGI
- abnormal osteoclast cell number / MGI
- decreased osteoclast cell number / MGI
- increased eosinophil cell number / MGI
- increased B cell number / MGI
- abnormal wound healing / MGI
- diarrhea / MGI
- decreased acute inflammation / MGI
- decreased T cell proliferation / MGI
- abnormal optic choroid morphology / MGI
- abnormal retinal pigment epithelium morphology / MGI
- abnormal Bruch membrane morphology / MGI
- abnormal cholesterol homeostasis / MGI
- abnormal renal glomerulus morphology / MGI
- abnormal podocyte morphology / MGI
- abnormal Langerhans cell physiology / MGI
- immune system phenotype / MGI
- choroidal neovascularization / MGI
- decreased circulating creatinine level / MGI
- decreased susceptibility to type I hypersensitivity reaction / MGI
- retinal deposits / MGI
- abnormal choriocapillaris morphology / MGI
- abnormal urine protein level / MGI
- decreased CD4-positive, alpha beta T cell number / MGI
- increased CD8-positive, alpha-beta T cell number / MGI
- increased dendritic cell number / MGI
- abnormal osteoclast differentiation / MGI
- retinal photoreceptor degeneration / MGI
- retinal outer nuclear layer degeneration / MGI
- increased susceptibility to induced colitis / MGI
- decreased susceptibility to induced colitis / MGI
- increased tumor necrosis factor secretion / MGI
- increased interferon-gamma secretion / MGI
- decreased interferon-gamma secretion / MGI
- decreased interleukin-2 secretion / MGI
- decreased interleukin-6 secretion / MGI
- abnormal chemokine secretion / MGI
- decreased common myeloid progenitor cell number / MGI
- lipofuscinosis / MGI
- increased physiological sensitivity to xenobiotic / MGI
- decreased splenocyte proliferation / MGI
- increased trabecular bone thickness / MGI
- increased susceptibility to viral infection induced morbidity/mortality / MGI
- abnormal cellular extravasation / MGI
- decreased susceptibility to bone fracture / MGI
- abnormal bone mineral density / MGI
- abnormal dendritic cell chemotaxis / MGI
- abnormal macrophage chemotaxis / MGI
- abnormal bone trabecula morphology / MGI
- increased trabecular bone volume / MGI
- increased fluid intake / MGI
- increased CD11b-high dendritic cell number / MGI
- decreased CD11b-high dendritic cell number / MGI
- impaired leukocyte tethering or rolling / MGI
- altered response to myocardial infarction / MGI
- abnormal locomotor behavior / MGI
- abnormal spatial learning / MGI
- abnormal cardiovascular system physiology / MGI
- decreased inflammatory response / MGI
- decreased susceptibility to viral infection / MGI
- increased susceptibility to bacterial infection / MGI
- abnormal CD4-positive, alpha beta T cell morphology / MGI
- abnormal leukocyte physiology / MGI
- abnormal conditioned taste aversion behavior / MGI
- abnormal cytokine secretion / MGI
- liver failure / MGI
- increased alcohol consumption / MGI
- abnormal leukocyte adhesion / MGI
- nervous system phenotype / MGI
- abnormal nervous system physiology / MGI
- abnormal glial cell physiology / MGI
- impaired macrophage chemotaxis / MGI
- increased length of allograft survival / MGI
- abnormal CD8-positive, alpha beta T cell morphology / MGI
- decreased T cell proliferation / MGI
- decreased susceptibility to injury / MGI
- cardiovascular system phenotype / MGI
- behavior/neurological phenotype / MGI
- reproductive system phenotype / MGI
- increased susceptibility to fungal infection / MGI
- abnormal heart size / MGI
- corneal vascularization / MGI
- increased susceptibility to type IV hypersensitivity reaction / MGI
- increased NK T cell number / MGI
- abnormal NK T cell physiology / MGI
- decreased NK cell number / MGI
- abnormal Kupffer cell morphology / MGI
- abnormal long term spatial reference memory / MGI
- increased IgG1 level / MGI
- decreased susceptibility to induced colitis / MGI
- increased interferon-gamma secretion / MGI
- decreased interleukin-1 beta secretion / MGI
- increased interleukin-4 secretion / MGI
- decreased interleukin-6 secretion / MGI
- abnormal cytokine level / MGI
- decreased susceptibility to endotoxin shock / MGI
- increased physiological sensitivity to xenobiotic / MGI
- abnormal astrocyte physiology / MGI
- abnormal Ito cell morphology / MGI
- abnormal cellular extravasation / MGI
- abnormal hepatocyte physiology / MGI
- decreased right ventricle systolic pressure / MGI
- mortality/aging / MGI
- increased fluid intake / MGI
- decreased microglial cell activation / MGI
Literature references
- Chemokine receptor Ccr2 deficiency reduces renal disease and prolongs survival in MRL/lpr lupus-prone mice.;Pérez de Lema Guillermo, Maier Holger, Franz Tobias J, Escribese Maríia, Chilla Silvia, Segerer Stephan, Camarasa Natalia, Schmid Holger, Banas Bernhard, Kalaydjiev Svetoslav, Busch Dirk H, Pfeffer Klaus, Mampaso Francisco, Schlöndorff Detlef, Luckow Bruno, ;2005;Journal of the American Society of Nephrology : JASN;16;3592-601; 16267157
- Microinjection of Cre recombinase protein into zygotes enables specific deletion of two eukaryotic selection cassettes and enhances the expression of a DsRed2 reporter gene in Ccr2/Ccr5 double-deficient mice.;Luckow Bruno, Hänggli Amy, Maier Holger, Chilla Silvia, Loewe Robert P, Dehmel Stefan, Schlöndorff Detlef, Loetscher Pius, Zerwes Hans-Günter, Müller Matthias, ;2009;Genesis (New York, N.Y. : 2000);47;545-58; 19517561