B6.129-Unc13atm1Bros/Ph

Status

Available to order

EMMA IDEM:11244
Citation informationRRID:IMSR_EM:11244 

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International strain nameB6.129-Unc13atm1Bros/Ph
Alternative nameUnc13a tm1Bros
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolUnc13atm1Bros
Gene/Transgene symbolUnc13a

Information from provider

ProviderNils Brose
Provider affiliationDepartment of Molecular Neurobiology, Max Planck Institute of Experimental Medicine
Genetic informationIn the targeting vector, four exons, representing base pairs 2587 to 2964 of rat Munc13-1, were replaced with a neomycin resistance gene. Upon homologous recombination of the targeting vector with the Munc13-1 gene, the insertion of the neomycin resistance cassette results in the deletion of amino-acid residues 494 to 618 (including the C1 domain) and a shift in the open reading frame of the mature messenger RNA.
Phenotypic informationHomozygous:
Munc13-1-deficient mice do not feed and are distinguishable from wild-type animals shortly after birth by a lack of milk in their stomachs, weakness and reduced breathing rate. Mutant mice die within a few hours of birth.

Heterozygous:
Heterozygous mice are normal and show no obvious alterations in comparison to wild-type controls.
Breeding historyHomologous recombination in 129/Sv embryonic stem cells, consecutive backcrosses with C57BL/6J animals.
References
  • Munc13-1 is essential for fusion competence of glutamatergic synaptic vesicles.;Augustin I, Rosenmund C, Südhof T C, Brose N, ;1999;Nature;400;457-61; 10440375
Homozygous fertileno
Homozygous viableno
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreInstitute of Molecular Genetics, Prague, Czech Republic
Animals used for archivingheterozygous C57BL/6 x 129/Sv males

Disease and phenotype information

MGI phenotypes (allele matching)
  • decreased insulin secretion / MGI
  • abnormal pancreatic islet morphology / MGI
  • impaired glucose tolerance / MGI
  • weakness / MGI
  • abnormal CNS synaptic transmission / MGI
  • nervous system phenotype / MGI
  • decreased neurotransmitter release / MGI
  • abnormal synaptic vesicle number / MGI
  • decreased pulmonary respiratory rate / MGI
  • absent gastric milk in neonates / MGI
  • postnatal lethality, complete penetrance / MGI
  • neonatal lethality, complete penetrance / MGI
MGI phenotypes (gene matching)
  • weakness / MGI
  • abnormal CNS synaptic transmission / MGI
  • abnormal excitatory postsynaptic currents / MGI
  • decreased synaptic depression / MGI
  • decreased insulin secretion / MGI
  • nervous system phenotype / MGI
  • decreased neurotransmitter release / MGI
  • abnormal synaptic vesicle morphology / MGI
  • abnormal synaptic vesicle recycling / MGI
  • abnormal synaptic vesicle number / MGI
  • abnormal neuron physiology / MGI
  • abnormal pancreatic islet morphology / MGI
  • impaired glucose tolerance / MGI
  • homeostasis/metabolism phenotype / MGI
  • reproductive system phenotype / MGI
  • decreased pulmonary respiratory rate / MGI
  • absent gastric milk in neonates / MGI
  • postnatal lethality, complete penetrance / MGI
  • neonatal lethality, complete penetrance / MGI

Literature references

  • Munc13-1 is essential for fusion competence of glutamatergic synaptic vesicles.;Augustin I, Rosenmund C, Südhof T C, Brose N, ;1999;Nature;400;457-61; 10440375

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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