FVB-Tg(Thy1-GFP*)1Rfc/Cnbc
| Status | Available to order |
| EMMA ID | EM:11430 |
| Citation information | RRID:IMSR_EM:11430 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | FVB-Tg(Thy1-GFP*)1Rfc/Cnbc |
| Alternative name | FVB-Tg(Thy-1-SypHA)1RFC/US |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Thy1-GFP*)1Rfc |
| Gene/Transgene symbol | Tg(Thy1-GFP*)1Rfc |
Information from provider
| Provider | Rafael Fernández-Chacón |
| Provider affiliation | Universidad de Sevilla |
| Genetic information | These mice express, in motor nerve terminals, synaptopHluorin, a vesicular pH-sensitive fluorescent reporter of exo-endocytosis. At the neuromuscular junction (NMJ) of synaptopHluorin transgenic mice, exocytosis is followed by compensatory endocytosis that can be monitored as a fluorescence decay after the electrical stimulation train stops. |
| Phenotypic information | Homozygous:No apparent phenotype, not increased mortality, not increased morbidity.Heterozygous:No apparent phenotype, not increased mortality, not increased morbidity. |
| Breeding history | The transgenic founders were intercrossed to obtain homozygous transgenic mice. The homozygous line is maintained inbred. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous FVB males, homozygous FVB females |
| Stage of embryos | 2-cell |
Literature references
- Monitoring synaptic function at the neuromuscular junction of a mouse expressing synaptopHluorin.;Tabares Lucia, Ruiz Rocio, Linares-Clemente Pedro, Gaffield Michael A, Alvarez de Toledo Guillermo, Fernandez-Chacón Rafael, Betz William J, ;2007;The Journal of neuroscience : the official journal of the Society for Neuroscience;27;5422-30; 17507564
- Different dynamin blockers interfere with distinct phases of synaptic endocytosis during stimulation in motoneurones.;Linares-Clemente Pedro, Rozas José L, Mircheski Josif, García-Junco-Clemente Pablo, Martínez-López José A, Nieto-González José L, Vázquez M Eugenio, Pintado C Oscar, Fernández-Chacón Rafael, ;2015;The Journal of physiology;593;2867-88; 25981717