FVB.Cg-Dnajc5tm1Sud Tg(Thy1-GFP*)1Rfc/Cnbc
| Status | Available to order |
| EMMA ID | EM:11431 |
| Citation information | RRID:IMSR_EM:11431 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | FVB.Cg-Dnajc5tm1Sud Tg(Thy1-GFP*)1Rfc/Cnbc |
| Alternative name | FVB.Cg-Tg(Thy-1-SypHA)1RFC Dnajc5 Tm1Sud/US |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Dnajc5tm1Sud, Tg(Thy1-GFP*)1Rfc |
| Gene/Transgene symbol | Dnajc5, Tg(Thy1-GFP*)1Rfc |
Information from provider
| Provider | Rafael Fernández-Chacón |
| Provider affiliation | Universidad de Sevilla |
| Genetic information | Gene symbol: Dnajc5; protein synonym: CSP-alpha. Cysteine String Protein-alpha (CSP-alpha) is a synaptic vesicle protein that prevents activity-dependent synaptic degeneration. The mutant mice develop a strong neurological phenotype that causes early lethality within 30-40 days of age. These mice express the synaptic vesicle cycle reporter synaptopHluorin at motor nerve terminals. |
| Phenotypic information | Homozygous:Newborn homozygotes with this targeted mutation are viable, normal in size, and do not display any gross physical or behavioral abnormalities. After 15 days of age, the animals stop gaining weight. At 3-4 weeks of age, these mice develop a progressive, fatal sensorimotor disorder.Heterozygous:No apparent phenotype, not increased mortality, not increased morbidity. |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6.FVB males, heterozygous C57BL/6.FVB females |
| Stage of embryos | 2-cell |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- CLN4B disease / Orphanet_228343
Literature references
- Motorneurons require cysteine string protein-α to maintain the readily releasable vesicular pool and synaptic vesicle recycling.;Rozas José Luis, Gómez-Sánchez Leonardo, Mircheski Josif, Linares-Clemente Pedro, Nieto-González José Luis, Vázquez M Eugenio, Luján Rafael, Fernández-Chacón Rafael, ;2012;Neuron;74;151-65; 22500637
- The synaptic vesicle protein CSP alpha prevents presynaptic degeneration.;Fernández-Chacón Rafael, Wölfel Markus, Nishimune Hiroshi, Tabares Lucia, Schmitz Frank, Castellano-Muñoz Manuel, Rosenmund Christian, Montesinos Maria L, Sanes Joshua R, Schneggenburger Ralf, Südhof Thomas C, ;2004;Neuron;42;237-51; 15091340
- Different dynamin blockers interfere with distinct phases of synaptic endocytosis during stimulation in motoneurones.;Linares-Clemente Pedro, Rozas José L, Mircheski Josif, García-Junco-Clemente Pablo, Martínez-López José A, Nieto-González José L, Vázquez M Eugenio, Pintado C Oscar, Fernández-Chacón Rafael, ;2015;The Journal of physiology;593;2867-88; 25981717
- Monitoring synaptic function at the neuromuscular junction of a mouse expressing synaptopHluorin.;Tabares Lucia, Ruiz Rocio, Linares-Clemente Pedro, Gaffield Michael A, Alvarez de Toledo Guillermo, Fernandez-Chacón Rafael, Betz William J, ;2007;The Journal of neuroscience : the official journal of the Society for Neuroscience;27;5422-30; 17507564