IMPC phenotypes (gene matching)
B6N;129P2-Nox4tm1.2Hwsc/Orl
| Status | Available to order |
| EMMA ID | EM:11551 |
| Citation information | RRID:IMSR_EM:11551 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6N;129P2-Nox4tm1.2Hwsc/Orl |
| Alternative name | C57Bl/6N-Nox4tm1.2Hwsc |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Nox4tm1.2Hwsc |
| Gene/Transgene symbol | Nox4 |
Information from provider
| Provider | Harald Schmidt |
| Provider affiliation | Department of Pharmacology & Personalised Medicine, Maastricht University |
| Genetic information | The Nox4 gene is flanked by loxP sites around exons 14 and 15; cre-loxP technology, type 1 mutation for conditional knock-outs. |
| Phenotypic information | Homozygous:No spontaneous phenotype.Heterozygous:Not applicable. |
| Breeding history | Recombinant ES-cell clones were injected into wild-type (C57BL/6) blastocysts. Successfully transfected embryos were then transferred into the uterus of mock-pregnant mice. The resulting chimeras were then mated with C57BL/6 females and the F1 offspring tested for heterozygosity. F1 males were mated with two C57BL/6 females each. Of the resulting offspring 10 males were genotyped by microsatellite analysis of 110 different polymorphic markers (Charles River MAX-BAXSM analysis). Males showing the highest homology to the wild-type C57BL/6 strain were then mated again. After the content of recipient C57BL/6 genome was highest than 95%, the mice were intercrossed to gain homozygous offspring. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | not known |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6N males, heterozygous C57BL/6N females |
| Stage of embryos | 2-cell |
Disease and phenotype information
MGI phenotypes (gene matching)
- abnormal lung morphology / MGI
- cardiac hypertrophy / MGI
- abnormal kidney physiology / MGI
- no phenotypic analysis / MGI
- abnormal redox activity / MGI
- decreased neuron apoptosis / MGI
- oxidative stress / MGI
- increased response of heart to induced stress / MGI
- dilated heart / MGI
- abnormal blood-brain barrier function / MGI
- cardiovascular system phenotype / MGI
- cardiac interstitial fibrosis / MGI
- decreased apoptosis / MGI
- decreased cerebral infarction size / MGI
- decreased susceptibility to ischemic brain injury / MGI
- decreased physiological sensitivity to xenobiotic / MGI
- decreased susceptibility to weight loss / MGI
Literature references
- NOX4-dependent neuronal autotoxicity and BBB breakdown explain the superior sensitivity of the brain to ischemic damage.;Casas Ana I, Geuss Eva, Kleikers Pamela W M, Mencl Stine, Herrmann Alexander M, Buendia Izaskun, Egea Javier, Meuth Sven G, Lopez Manuela G, Kleinschnitz Christoph, Schmidt Harald H H W, ;2017;Proceedings of the National Academy of Sciences of the United States of America;114;12315-12320; 29087944
- Post-stroke inhibition of induced NADPH oxidase type 4 prevents oxidative stress and neurodegeneration.;Kleinschnitz Christoph, Grund Henrike, Wingler Kirstin, Armitage Melanie E, Jones Emma, Mittal Manish, Barit David, Schwarz Tobias, Geis Christian, Kraft Peter, Barthel Konstanze, Schuhmann Michael K, Herrmann Alexander M, Meuth Sven G, Stoll Guido, Meurer Sabine, Schrewe Anja, Becker Lore, Gailus-Durner Valérie, Fuchs Helmut, Klopstock Thomas, de Angelis Martin Hrabé, Jandeleit-Dahm Karin, Shah Ajay M, Weissmann Norbert, Schmidt Harald H H W, ;2010;PLoS biology;8;; 20877715