B6J.B6-Tg(Kit*D814V)1Roer/Ph
| Status | Available to order |
| EMMA ID | EM:11567 |
| Citation information | RRID:IMSR_EM:11567 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6J.B6-Tg(Kit*D814V)1Roer/Ph |
| Alternative name | B6 Tg(Kit*D814V)1Roer |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Kit*D814V)1Roer |
| Gene/Transgene symbol | Tg(Kit*D814V)1Roer |
Information from provider
| Provider | Axel Roers |
| Provider affiliation | Institute for Immunology, Medical Faculty TU Dresden |
| Genetic information | Mutation details: This transgene consists of a modified mouse BAC clone RP23-274L11 which contains the entire Kit locus. The Kit locus in the BAC was modified by the insertion of a floxed stop cassette into intron 1 and the insertion of an A to T point mutation in exon 17 resulting in an aspartic acid to valine mutation at position 814. This mutation is homologous to the D816V mutation in humans. This line carries 5-6 copies of the transgene. |
| Phenotypic information | Homozygous:No phenotype without cre recombinase expression. Homozygous Tg(Kit*D814V)1Roer mice are viable and fertile.Heterozygous:No phenotype without cre recombinase expression. Hemizygous Tg(Kit*D814V)1Roer mice are viable and fertile. Mating of Tg(Kit*D814V)1Roer to Mx1-cre leads to mastocytosis in skin and internal organs, pre B lymphocyte neoplasia and colitis. Mating of Tg(Kit*D814V)1Roer to Cd19-cre results in pre B lymphocyte neoplasia. Mating of Tg(Kit*D814V)1Roer to Cma1-cre (Mcpt5-cre) leads to mastocytosis and colitis. |
| Breeding history | Pronucleus injection into C57BL/6 oocytes yielded several founder lines and was performed in 2006. Tg1 founder line was constantly backcrossed to C57BL/6 since 2006 (>20 generations). Tg(Kit*D814V)1Roer hemizygous parents are currently bred to each other. C57BL/6JRj from Janvier was used for backcrossing since 2010. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institute of Molecular Genetics, Prague, Czech Republic |
| Animals used for archiving | homozygous C57BL/6J males |
Literature references
- Mast cell hyperplasia, B-cell malignancy, and intestinal inflammation in mice with conditional expression of a constitutively active kit.;Gerbaulet Alexander, Wickenhauser Claudia, Scholten Julia, Peschke Katrin, Drube Sebastian, Horny Hans-Peter, Kamradt Thomas, Naumann Ronald, Müller Werner, Krieg Thomas, Waskow Claudia, Hartmann Karin, Roers Axel, ;2011;Blood;117;2012-21; 21148330