IMPC phenotypes (gene matching)
B6.129P2-Unc13ctm1Bros/Ph
| Status | Available to order |
| EMMA ID | EM:11617 |
| Citation information | RRID:IMSR_EM:11617 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129P2-Unc13ctm1Bros/Ph |
| Alternative name | Unc13c tm1Bros |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Unc13ctm1Bros |
| Gene/Transgene symbol | Unc13c |
Information from provider
| Provider | Nils Brose |
| Provider affiliation | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine |
| Genetic information | In the targeting vector, two exons representing bp 3108 –3265 of the rat Munc13-3 cDNA (GenBank accession number U24072) were replaced by a neomycin resistance cassette. The vector also contained two copies of the herpes simplex virus thymidine kinase gene. Upon homologous recombination of the targeting vector with the Munc13-3 gene, the insertion of the neomycin resistance cassette results in a deletion that represents amino acid residues 1014 –1066 (upstream of the C1 domain) and a shift in the open reading frame in the mature mRNA. |
| Phenotypic information | Homozygous:Munc 13-3 KOs exhibit no obvious abnormalities and have a normal life expectancy. Both male and female Munc13-3 KOs are fertile. Litter sizes resulting from interbreeding of homozygous Munc13-3 KOs are indistinguishable from those obtained with wild-type controls. KOs have an impaired ability to learn complex motor tasks.Heterozygous:Heterozygous mice are normal and show no obvious alterations in comparison to wild-type controls. |
| Breeding history | Homologous recombination in 129/Sv embryonic stem cells, consecutive backcrosses with C57BL/6J animals. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institute of Molecular Genetics, Prague, Czech Republic |
| Animals used for archiving | homozygous 129/Sv x C57BL/6J males |
| Breeding at archiving centre | 17 backcrosses to C57BL/6J |
Disease and phenotype information
Literature references
- The cerebellum-specific Munc13 isoform Munc13-3 regulates cerebellar synaptic transmission and motor learning in mice.;Augustin I, Korte S, Rickmann M, Kretzschmar H A, Südhof T C, Herms J W, Brose N, ;2001;The Journal of neuroscience : the official journal of the Society for Neuroscience;21;10-7; 11150314