IMPC phenotypes (gene matching)
B6.Cg-Sdhbtm1Dost/H
| Status | Available to order |
| EMMA ID | EM:11640 |
| Citation information | RRID:IMSR_EM:11640 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Sdhbtm1Dost/H |
| Alternative name | Sdhb-tm1Dost |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Sdhbtm1Dost |
| Gene/Transgene symbol | Sdhb |
Information from provider
| Provider | Douglas Strathdee |
| Provider affiliation | CRUK Beatson Institute |
| Genetic information | A hygromycin resistance cassette with a 3' loxP site was inserted upstream of exon 3. An additional loxP site was inserted downstream of exon 3. |
| Phenotypic information | Homozygous:Homozygous floxed mice are viable, fertile and have a normal lifespan. However after cre recombination homozygous knockouts may be infertile.Heterozygous:Heterozygous floxed mice are viable, fertile and have a normal lifespan. |
| Breeding history | Mice were crossed to a strain containing the Tg(ACTFLPe)9205Dym transgene. Expression of Flp recombinase was used to remove the hygromycin resistance gene. Subsequent backcrossing to C57BL/6J allowed the segregation of the flox allele. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
| Animals used for archiving | heterozygous C57BL/6J males |
| Breeding at archiving centre | Backcrossed onto C57BL/6J for 10 generations |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Carney-Stratakis syndrome / Orphanet_97286
- Hereditary pheochromocytoma-paraganglioma / Orphanet_29072
- Isolated succinate-CoQ reductase deficiency / Orphanet_3208
- Cowden syndrome / Orphanet_201
Literature references
- Pyruvate carboxylation enables growth of SDH-deficient cells by supporting aspartate biosynthesis.;Cardaci Simone, Zheng Liang, MacKay Gillian, van den Broek Niels J F, MacKenzie Elaine D, Nixon Colin, Stevenson David, Tumanov Sergey, Bulusu Vinay, Kamphorst Jurre J, Vazquez Alexei, Fleming Stewart, Schiavi Francesca, Kalna Gabriela, Blyth Karen, Strathdee Douglas, Gottlieb Eyal, ;2015;Nature cell biology;17;1317-26; 26302408