B6.129P2-Car9^tm1Spas/Oulu

Status	Available to order
EMMA ID	EM:11726
Citation information	RRID:IMSR_EM:11726 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.129P2-Car9^tm1Spas/Oulu
Alternative name	Car9tm1Spas
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Car9^tm1Spas
Gene/Transgene symbol	Car9

Information from provider

Provider	Seppo Parkkila
Provider affiliation	Medicine and Life Sciences, University of Tampere
Additional owner	Prof. Ivan Horak, FU Berlin/Charité, Berlin, Germany.
Genetic information	A PGK-neo cassette was inserted into exon 1 of Car9 gene by homologous recombination. The insertion of neo downstream of the signal peptide deleted 14 nt (268-282) of the proteoglycan domain and caused a frameshift mutation.
Phenotypic information	Homozygous: Gastric hyperplasia, abnormal digestive system physiology. Heterozygous: NA
References	Gastric hyperplasia in mice with targeted disruption of the carbonic anhydrase gene Car9.;Gut Marta Ortova, Parkkila Seppo, Vernerová Zdeòka, Rohde Elvira, Závada Jan, Höcker Michael, Pastorek Jaromír, Karttunen Tuomo, Gibadulinová Adriana, Závadová Zuzana, Knobeloch Klaus-Peter, Wiedenmann Bertram, Svoboda Jan, Horak Ivan, Pastoreková Silvia, ;2002;Gastroenterology;123;1889-903; 12454846 Gastric pit cell hyperplasia and glandular atrophy in carbonic anhydrase IX knockout mice: studies on two strains C57/BL6 and BALB/C.;Leppilampi Mari, Karttunen Tuomo J, Kivelä Jyrki, Gut Marta Ortova, Pastoreková Silvia, Pastorek Jaromir, Parkkila Seppo, ;2005;Transgenic research;14;655-63; 16245156 Carbonic anhydrase gene expression in CA II-deficient (Car2-/-) and CA IX-deficient (Car9-/-) mice.;Pan Peiwen, Leppilampi Mari, Pastorekova Silvia, Pastorek Jaromir, Waheed Abdul, Sly William S, Parkkila Seppo, ;2006;The Journal of physiology;571;319-27; 16396925 Global transcriptional response to carbonic anhydrase IX deficiency in the mouse stomach.;Kallio Heini, Hilvo Mika, Rodriguez Alejandra, Lappalainen Eeva-Helena, Lappalainen Anna-Maria, Parkkila Seppo, ;2010;BMC genomics;11;397; 20573196 Brain phenotype of carbonic anhydrase IX-deficient mice.;Pan Pei-wen, Parkkila Anna-Kaisa, Autio Salla, Hilvo Mika, Sormunen Raija, Pastorekova Silvia, Pastorek Jaromir, Haapasalo Hannu, Parkkila Seppo, ;2012;Transgenic research;21;163-76; 21547424
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	University of Oulu, Oulu, Finland
Animals used for archiving	heterozygous C57BL/6JOlaHsd males, wild-type C57BL/6NCrl females
Stage of embryos	2-cell

Disease and phenotype information

MGI phenotypes (gene matching)

abnormal digestive system physiology / MGI
duodenal lesions / MGI
abnormal gastric parietal cell morphology / MGI
abnormal small intestine crypts of Lieberkuhn morphology / MGI
digestive/alimentary phenotype / MGI
stomach epithelial hyperplasia / MGI
increased mature B cell number / MGI
abnormal stomach mucosa morphology / MGI
gastric gland atrophy / MGI

Literature references

Gastric hyperplasia in mice with targeted disruption of the carbonic anhydrase gene Car9.;Gut Marta Ortova, Parkkila Seppo, Vernerová Zdeòka, Rohde Elvira, Závada Jan, Höcker Michael, Pastorek Jaromír, Karttunen Tuomo, Gibadulinová Adriana, Závadová Zuzana, Knobeloch Klaus-Peter, Wiedenmann Bertram, Svoboda Jan, Horak Ivan, Pastoreková Silvia, ;2002;Gastroenterology;123;1889-903; 12454846
Gastric pit cell hyperplasia and glandular atrophy in carbonic anhydrase IX knockout mice: studies on two strains C57/BL6 and BALB/C.;Leppilampi Mari, Karttunen Tuomo J, Kivelä Jyrki, Gut Marta Ortova, Pastoreková Silvia, Pastorek Jaromir, Parkkila Seppo, ;2005;Transgenic research;14;655-63; 16245156
Carbonic anhydrase gene expression in CA II-deficient (Car2-/-) and CA IX-deficient (Car9-/-) mice.;Pan Peiwen, Leppilampi Mari, Pastorekova Silvia, Pastorek Jaromir, Waheed Abdul, Sly William S, Parkkila Seppo, ;2006;The Journal of physiology;571;319-27; 16396925
Global transcriptional response to carbonic anhydrase IX deficiency in the mouse stomach.;Kallio Heini, Hilvo Mika, Rodriguez Alejandra, Lappalainen Eeva-Helena, Lappalainen Anna-Maria, Parkkila Seppo, ;2010;BMC genomics;11;397; 20573196
Brain phenotype of carbonic anhydrase IX-deficient mice.;Pan Pei-wen, Parkkila Anna-Kaisa, Autio Salla, Hilvo Mika, Sormunen Raija, Pastorekova Silvia, Pastorek Jaromir, Haapasalo Hannu, Parkkila Seppo, ;2012;Transgenic research;21;163-76; 21547424

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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B6.129P2-Car9tm1Spas/Oulu