MGI phenotypes (allele matching)
- prenatal lethality, complete penetrance / MGI
D1.B6-Tg(CAG-EGFP)1Osb/JOrl
| Status | Available to order |
| EMMA ID | EM:11813 |
| Citation information | RRID:IMSR_EM:11813 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | D1.B6-Tg(CAG-EGFP)1Osb/JOrl |
| Alternative name | D1.B6-Tg(CAG-EGFP)1Osb/J |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(CAG-EGFP)1Osb |
| Gene/Transgene symbol | Tg(CAG-EGFP)1Osb |
Information from provider
| Provider | Pascale Plence |
| Provider affiliation | INSERM U1183 |
| Genetic information | cDNA encoding enhanced green fluorescent protein (EGFP) was adjoined to the chicken beta actin promoter and cytomegalovirus enhancer. A bovine globin polyadenylation signal was also included in the construct. Submitted mouse strain was obtained by backcrossing strain C57BL/6-Tg(CAG-EGFP)1Osb/J, obtained by the Jackson Laboratory (003291), for 23 generations on DBA/1 background (SNP analysis found 99.17% homology with DBA/1OlaHsd used in the backcrosses). |
| Phenotypic information | Homozygous:Homozygous mice are not viable.Heterozygous:This strain was maintained by mating a hemizygous carrier with a DBA/1 mouse. This transgenic mouse line, with an "enhanced" GFP (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer, have widespread EGFP fluorescence, with the exception of erythrocytes and hair. |
| Breeding history | 23 backcrosses on DBA/1 background |
| References | None available |
| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | heterozygous DBA/1 males |
Disease and phenotype information
MGI phenotypes (gene matching)
- prenatal lethality, complete penetrance / MGI