IMPC phenotypes (gene matching)
B6.129-Itga11tm1Dgul/Kctt
| Status | Available to order |
| EMMA ID | EM:11843 |
| Citation information | RRID:IMSR_EM:11843 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129-Itga11tm1Dgul/Kctt |
| Alternative name | B6.Cg-Itga11KO Dgul |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Itga11tm1Dgul |
| Gene/Transgene symbol | Itga11 |
Information from provider
| Provider | Donald Gullberg |
| Provider affiliation | Biomedicine, University of Bergen |
| Genetic information | Integrin a11 forms heterodimers exclusively with integrin b1. Integrin a11b1 is cell-surface collagen receptor that mediate cell-matrix interactions. The homozygous Itga11KO strain has null mutation in both integrin a11 alleles and have the phenotype of tooth eruption deficiency which leads to smaller size in the homozygotes comparing with their wildtype and heterozygous littermates. |
| Phenotypic information | Homozygous:The homozygous Itga11KO strain has null mutation in both integrin a11 alleles and the phenotype with tooth eruption deficiency, which leads to smaller size in the homozygotes comparing with their wildtype and heterozygous littermates. The phenotype can partially be rescued by feeding the homozygotes with soft food. Both the homozygous male and female are fertile but the homozygous females tend to give birth to smaller litters (3-5 pups comparing with 8-10 pups from wildtype or heterozygous females).Heterozygous:No obvious phenotype. Behave similarly as wildtype. |
| Breeding history | The Itga11KO strain was generated by conventional gene targeting method, where targeted ES clones (D3 ES line) containing interrupted Itga11 gene (by deleting exon 3) were microinjected into C57BL/6J blastocysts and transferred to surrogate mothers. Chimeric male founder mice were crossed with C57BL/6J females to obtain heterozygous F1. Homozygous integrin a11-deficient (Itga11KO) mice were established by backcrossing F1 with C57BL/6J mice and intercrossing heterozygous offspring. The Itga11KO strain was first established around 2004 and the description on strain generation and characterizations was first published in 2007 (Popova S.N. et al, Mol Cell Biol. 2007). The strain has been kept by het*het intercross breeding. The strain has also been backcrossed to parental C57BL/6J strain a few times to minimize genetic drift. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | homozygous C57BL/6J males |
Disease and phenotype information
MGI phenotypes (gene matching)
- decreased cell proliferation / MGI
- decreased body weight / MGI
- premature death / MGI
- abnormal tooth morphology / MGI
- abnormal enamel morphology / MGI
- abnormal dentin morphology / MGI
- delayed tooth eruption / MGI
- abnormal cell adhesion / MGI
- abnormal periodontal ligament morphology / MGI
- short incisors / MGI
- abnormal incisor morphology / MGI
- abnormal food intake / MGI
- enamel pits / MGI
- increased number of epithelial cell rests of Malassez / MGI
Literature references
- Alpha11 beta1 integrin-dependent regulation of periodontal ligament function in the erupting mouse incisor.;Popova Svetlana N, Barczyk Malgorzata, Tiger Carl-Fredrik, Beertsen Wouter, Zigrino Paola, Aszodi Attila, Miosge Nicolai, Forsberg Erik, Gullberg Donald, ;2007;Molecular and cellular biology;27;4306-16; 17420280
- The mesenchymal alpha11beta1 integrin attenuates PDGF-BB-stimulated chemotaxis of embryonic fibroblasts on collagens.;Popova Svetlana N, Rodriguez-Sánchez Belén, Lidén Asa, Betsholtz Christer, Van Den Bos Theo, Gullberg Donald, ;2004;Developmental biology;270;427-42; 15183724
- Integrin alpha 11 regulates IGF2 expression in fibroblasts to enhance tumorigenicity of human non-small-cell lung cancer cells.;Zhu Chang-Qi, Popova Svetlana N, Brown Ewan R S, Barsyte-Lovejoy Dalia, Navab Roya, Shih Warren, Li Ming, Lu Ming, Jurisica Igor, Penn Linda Z, Gullberg Donald, Tsao Ming-Sound, ;2007;Proceedings of the National Academy of Sciences of the United States of America;104;11754-9; 17600088
- The alpha11beta1 integrin has a mechanistic role in control of interstitial fluid pressure and edema formation in inflammation.;Svendsen Ø S, Barczyk M M, Popova S N, Lidén A, Gullberg D, Wiig H, ;2009;Arteriosclerosis, thrombosis, and vascular biology;29;1864-70; 19729609
- Dwarfism in mice lacking collagen-binding integrins α2β1 and α11β1 is caused by severely diminished IGF-1 levels.;Blumbach Katrin, Niehoff Anja, Belgardt Bengt F, Ehlen Harald W A, Schmitz Markus, Hallinger Ralf, Schulz Jan-Niklas, Brüning Jens C, Krieg Thomas, Schubert Markus, Gullberg Donald, Eckes Beate, ;2012;The Journal of biological chemistry;287;6431-40; 22210772
- α11β1 integrin-mediated MMP-13-dependent collagen lattice contraction by fibroblasts: evidence for integrin-coordinated collagen proteolysis.;Barczyk Malgorzata M, Lu Ning, Popova Svetlana N, Bolstad Anne Isine, Gullberg Donald, ;2013;Journal of cellular physiology;228;1108-19; 23065814
- Reduced granulation tissue and wound strength in the absence of α11β1 integrin.;Schulz Jan-Niklas, Zeltz Cédric, Sørensen Ida W, Barczyk Malgorzata, Carracedo Sergio, Hallinger Ralf, Niehoff Anja, Eckes Beate, Gullberg Donald, ;2015;The Journal of investigative dermatology;135;1435-1444; 25634355
- Integrin α11β1 regulates cancer stromal stiffness and promotes tumorigenicity and metastasis in non-small cell lung cancer.;Navab R, Strumpf D, To C, Pasko E, Kim K S, Park C J, Hai J, Liu J, Jonkman J, Barczyk M, Bandarchi B, Wang Y H, Venkat K, Ibrahimov E, Pham N-A, Ng C, Radulovich N, Zhu C-Q, Pintilie M, Wang D, Lu A, Jurisica I, Walker G C, Gullberg D, Tsao M-S, ;2016;Oncogene;35;1899-908; 26148229
- Stromal integrin α11 regulates PDGFR-β signaling and promotes breast cancer progression.;Primac Irina, Maquoi Erik, Blacher Silvia, Heljasvaara Ritva, Van Deun Jan, Smeland Hilde Yh, Canale Annalisa, Louis Thomas, Stuhr Linda, Sounni Nor Eddine, Cataldo Didier, Pihlajaniemi Taina, Pequeux Christel, De Wever Olivier, Gullberg Donald, Noel Agnès, ;2019;The Journal of clinical investigation;129;4609-4628; 31287804
- New developments on skin fibrosis - Essential signals emanating from the extracellular matrix for the control of myofibroblasts.;Schulz Jan-Niklas, Plomann Markus, Sengle Gerhard, Gullberg Donald, Krieg Thomas, Eckes Beate, ;2018;Matrix biology : journal of the International Society for Matrix Biology;-6869;522-532; 29408278
- Integrin α11β1 is a receptor for collagen XIII.;Koivunen Jarkko, Tu Hongmin, Kemppainen Antti, Anbazhagan Padmanabhan, Finnilä Mikko A, Saarakkala Simo, Käpylä Jarmo, Lu Ning, Heikkinen Anne, Juffer André H, Heino Jyrki, Gullberg Donald, Pihlajaniemi Taina, ;2021;Cell and tissue research;383;1135-1153; 33306155