MGI phenotypes (gene matching)
C57BL/6NCrl-Slc25a32em1Orl/Orl
| Status | Available to order |
| EMMA ID | EM:11872 |
| Citation information | RRID:IMSR_EM:11872 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6NCrl-Slc25a32em1Orl/Orl |
| Alternative name | C57Bl6/NCrl |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Slc25a32em1Orl |
| Gene/Transgene symbol | Slc25a32 |
Information from provider
| Provider | Timothy Wai |
| Provider affiliation | Cell Biology and Infection, Institut Pasteur CNRS UMR 3691 |
| Genetic information | Crispr/Cas9 mediated 2 bp deletion by zygote injection of Cas9 protein and sgRNA causing premature stop codon (c. 413delAT) and loss of function of Slc25a32. Depletion of Slc25a32 confirmed by Western blot in tissue lysates of heterozygous mutant mice. Germline transmission of the mutant allele confirmed. |
| Phenotypic information | Homozygous:Inability to generate homozygous mutant mice by F1 intercross, indicative of embryonic lethality. Heterozygous:No obvious phenotypic abnormalities were observed in the first months of life. |
| Breeding history | Heterozygous mutant male mice were born to foster mothers following zygotic injection and embryo transfer. Germline transmission of the mutant allele by F1 intercross. |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6NCrl males |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Multiple acyl-CoA dehydrogenase deficiency, mild type / Orphanet_394532