B6.Cg-Tg(CAG-Col4a3bp)1Jsau/Cnbc
| Status | Available to order |
| EMMA ID | EM:11873 |
| Citation information | RRID:IMSR_EM:11873 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Tg(CAG-Col4a3bp)1Jsau/Cnbc |
| Alternative name | B6-HmGPBP |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(CAG-Col4a3bp)1Jsau |
| Gene/Transgene symbol | Tg(CAG-Col4a3bp)1Jsau |
Information from provider
| Provider | Juan Bautista Saus Mas |
| Provider affiliation | Bioquímica y Biología Molecular., Universidad de Valencia. Facultad de Medicina. |
| Genetic information | B6-HmGPBP are transgenic C57BL/6 mice that have undergone genomic insertion of mouse cDNA for Goodpasture antigen binding protein-1 (GPBP-1), also known as Cert-L/Cert1 (large isoform of ceramide transfer protein) or collagen type IV alpha-3-binding protein (Col4a3bp). B6-HmGPBP expresses high levels of mouse recombinant GPBP, apart from mouse endogenous GPBP. To generate this strain, we produced pCAGGS-mouseGPBP by inserting the mouse Col4a3bp cDNA into EcoRI site of pCAGGS expression vector (provided by Junichi Miyazaki, Osaka University Medical School, Osaka, Japan). A SnaBI-HindIII fragment of pCAGG-GPBP was subcloned into the pVAX vector (Invitrogen, Carlsbad, CA) to facilitate digestion with NruI and PmeI. The resulting DNA fragment (4.2 kb) was isolated and used for injection into the male pronucleus of fertilized oocytes obtained from a (C57BL/6 x DBA/2)F1 female mouse, where the fusion of pronuclei had not taken place yet. Afterwards, the resulting oocytes were transferred into the oviduct of a pseudopregnant NMRI female, prepared by mating with a sterile male. We screened the resulting offspring for transgene transmission by PCR analysis of genomic DNA extracted from mouse tails using mouse Col4a3bp specific primers. |
| Phenotypic information | Homozygous:There is no evident phenotype.Heterozygous:There is no evident phenotype. |
| Breeding history | Transgenic mice (F1, 50% C57BL/6 and 50% DBA/2) were backcrossed with C57BL/6 mice for six generations to acquire C57BL/6 genetic background (F2 to F7, 75 to 99.25% C57BL/6 and 25 to 0.75% DBA/2). |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6J males |