B6.Cg-Tg(CAG-COL4A3BP)2Jsau/Cnbc
| Status | Available to order |
| EMMA ID | EM:11912 |
| Citation information | RRID:IMSR_EM:11912 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Tg(CAG-COL4A3BP)2Jsau/Cnbc |
| Alternative name | B6-hGPBP |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(CAG-COL4A3BP)2Jsau |
| Gene/Transgene symbol | Tg(CAG-COL4A3BP)2Jsau |
Information from provider
| Provider | Juan Bautista Saus Mas |
| Provider affiliation | Bioquímica y Biología Molecular, Universidad de Valencia. Facultad de Medicina. |
| Genetic information | B6-hGPBP are transgenic C57BL/6 mice that have undergone genomic insertion of the cDNA for FLAG-tagged human Goodpasture antigen binding protein-1 (GPBP-1), also known as CERT-L/CERT1 (large isoform of ceramide transfer protein) or collagen type IV alpha-3-binding protein (COL4A3BP). To generate this strain, we produced pCAGGS-FLAG-GPBP-1 by inserting the cDNA encoding FLAG-tagged human GPBP-1 into EcoRI site of pCAGGS expression vector (provided by Junichi Miyazaki, Osaka University Medical School, Osaka, Japan). A SnaBI-HindIII fragment of pCAGG-FLAG-hGPBP-1 was subcloned into the pVAX1 vector (Invitrogen, Carlsbad, CA) generating pVAX1-pCAGGS-FLAG-hGPBP-1, that was subsequently digested with NruI and PmeI. The linearized DNA fragment was isolated and used for injection into the male pronucleus of fertilized oocytes obtained from a (C57BL/6 x DBA/2)F1 female mouse, where the fusion of pronuclei had not taken place yet. Afterwards, the resulting oocytes were transferred into the oviduct of a pseudopregnant NMRI female, prepared by mating with a sterile male. We screened the resulting offspring for transgene transmission by PCR analysis of genomic DNA extracted from mouse tails using specific GPBP primers. |
| Phenotypic information | Homozygous:Homozygous B6-hGPBP mice have not been obtained so far.Heterozygous:Heterozygous B6-hGPBP mice (homozygous mice have not been obtained) undergo type IV collagen disorganization and deposit of immunoglobulin A in glomerular basement membrane (Revert et al. 2007. Am J Pathol. 171:1419-30). B6-hGPBP mice express FLAG-tagged human GPBP-1. GPBP (Goodpasture antigen binding protein) is encoded by the COL4A3BP gene. The COL4A3BP pre-mRNA was shown to undergo alternative exon splicing which eliminates exon 11 and renders two mature mRNAs. One encoding the full primary product, called GPBP (Raya et al. 1999. J Biol Chem. 274: 12642–9) and an alternative polypeptide, called GPBPD26, devoid of 26-residue domain encoded by the exon 11 (Raya et al. 2000. J. Biol Chem. 275: 40392–9). GPBPD26 was further shown to transfer ceramide between reticulum endoplasmic and Golgi apparatus and it was renamed as CERT (Hanada et al. 2003. Nature. 426: 803–9). Further, the mRNA of GPBP was shown to undergo translation from canonical and non-canonical initiation sites, the latter being located at the 5’-untranslated region (5’-UTR), yielding two polypeptides: the primary canonical product (77-kDa GPBP) and previously unrecognized, non-canonical product (91-kDa GPBP). In contrast to GPBPD26/CERT, the 77-kDa GPBP was shown to undergo secretion and this to be enhanced by membrane-bound 91-kDa GPBP (Revert et al. 2008. J Biol Chem. 283:30246-55). B6-hGPBP mice express recombinant human FLAG-tagged GPBP-1, but not GPBP-3, so recombinant GPBP-1 secretion is limited. |
| Breeding history | Transgenic mice (F1, 50% C57BL/6 and 50% DBA/2) were backcrossed with C57BL/6 mice for six generations to acquire C57BL/6 genetic background (F2 to F7, 75 to 99.25% C57BL/6 and 25 to 0.75% DBA/2). |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6J males |
Literature references
- Increased Goodpasture antigen-binding protein expression induces type IV collagen disorganization and deposit of immunoglobulin A in glomerular basement membrane.;Revert Fernando, Merino Ramón, Monteagudo Carlos, Macias Jesús, Peydró Amando, Alcácer Javier, Muniesa Pedro, Marquina Regina, Blanco Mario, Iglesias Marcos, Revert-Ros Francisco, Merino Jesús, Saus Juan, ;2007;The American journal of pathology;171;1419-30; 17916599