B6.Cg-Dnaja3tm1.1Ukd/Ieg
| Status | Available to order |
| EMMA ID | EM:11920 |
| Citation information | RRID:IMSR_EM:11920 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Dnaja3tm1.1Ukd/Ieg |
| Alternative name | tidflox/KD-UMCM line 5 |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Dnaja3tm1.1Ukd |
| Gene/Transgene symbol | Dnaja3 |
Information from provider
| Provider | Ursula Kurzik-Dumke |
| Provider affiliation | Institut für Medizinische Mikrobiologie und Hygiene, Johannes Gutenberg-Universität Mainz |
| Genetic information | Homologous recombination of the floxed Tid (Dnaja3) allele, homozygous. To generate the conditional Tid (Dnaja3) knock-out line a targeting vector harbouring the mouse Tid genomic region, with a NeoR-cassette and FRT and loxP sites was generated. The genomic region was cloned into the pCR4BluntTopo vector. Exons 2 and 3 have been floxed, as a NeoR-cassette equipped with a loxP-FRT site (5’) and a FRT site (3’) has been introduced in intron 1 and a further loxP site has been located in intron 3. To generate the original Tid-flox line TC-1 (129S6/SvEvTac) ES cells (PolyGene AG, Riedmattstrasse 9, 8153 Rümlang, Schweiz) were used. Recombinant ES cells were injected into C57BL/6 albino blastocysts. For GLT chimeric males were mated with C57BL/6 albino females. Mutant offspring of this cross was further propagated by inbreeding. The conditional Tid knock-out line (line 4) was generated by flipping out of the NeoR-cassette, upon crossing it with Flpe deleter mice (B6;SJL-Tg(ACTFLPe)9205Dym/J strain). Line 5 originates from line 4, by backcrossing to the C57BL/6 background. |
| Phenotypic information | Homozygous:wild-typeHeterozygous:wild-type |
| Breeding history | 7 backcrosses, 3 inbred generations |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | homozygous C57BL/6J males |