B6;CBA-Tg(CAG-GFPuv/AEQ*)3Alns/Cnbc
| Status | Available to order |
| EMMA ID | EM:11953 |
| Citation information | RRID:IMSR_EM:11953 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;CBA-Tg(CAG-GFPuv/AEQ*)3Alns/Cnbc |
| Alternative name | erGAP3 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(CAG-GFPuv/AEQ*)3Alns |
| Gene/Transgene symbol | Tg(CAG-GFPuv/AEQ*)3Alns |
Information from provider
| Provider | María Teresa Alonso |
| Provider affiliation | IBGM-Universidad de Valladolid |
| Genetic information | The erGAP3 transgene comprises the calreticulin signal peptide and the sequence encoding the KDEL peptide for ER retention, fused in frame to the 3' and 5' end, respectively, of the joined GFP and aequorin reporter gene (GAP3; GenBank KX181639). |
| Phenotypic information | Homozygous:These transgenic mice express a new fluorescent calcium indicator based on the fusion of GFP and aequorin (GAP) into the endoplasmic reticulum. It becomes a valuable tool for in vivo Ca2+ imaging applications, especially in neuronal tissues.Heterozygous:These transgenic mice express a new fluorescent calcium indicator based on the fusion of GFP and aequorin (GAP) into the endoplasmic reticulum. It becomes a valuable tool for in vivo Ca2+ imaging applications, especially in neuronal tissues. |
| Breeding history | 7 generations of brother x sister matings have been performed. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous C57BL/6J males |
Literature references
- GAP, an aequorin-based fluorescent indicator for imaging Ca2+ in organelles.;Rodriguez-Garcia Arancha, Rojo-Ruiz Jonathan, Navas-Navarro Paloma, Aulestia Francisco Javier, Gallego-Sandin Sonia, Garcia-Sancho Javier, Alonso Maria Teresa, ;2014;Proceedings of the National Academy of Sciences of the United States of America;111;2584-9; 24501126
- GFP-Aequorin Protein Sensor for Ex Vivo and In Vivo Imaging of Ca(2+) Dynamics in High-Ca(2+) Organelles.;Navas-Navarro Paloma, Rojo-Ruiz Jonathan, Rodriguez-Prados Macarena, Ganfornina María Dolores, Looger Loren L, Alonso María Teresa, García-Sancho Javier, ;2016;Cell chemical biology;23;738-45; 27291400