B6N.Cg-Col1a1tm2(CAG-EGFP,-RNAi:Mirn26)Shzg/Biat

Status

Available to order

EMMA IDEM:12162
Citation informationRRID:IMSR_EM:12162 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

International strain nameB6N.Cg-Col1a1tm2(CAG-EGFP,-RNAi:Mirn26)Shzg/Biat
Alternative nameflox miR-26 sponge
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolCol1a1tm2(CAG-EGFP,-RNAi:Mirn26)Shzg
Gene/Transgene symbolCol1a1

Information from provider

ProviderSebastian Herzog
Provider affiliationDevelopmental Immunology, Medical University Innsbruck
Genetic informationFRT-mediated knock-in of a CAG-flox-stop-flox, EGFP reporter, miR-26 sponge/decoy cassette into the Col1a1 gene locus.
Phenotypic informationHomozygous:
Conditional mouse model; no phenotype unless flox-stop-flox-cassette is deleted by cre-mediated recombination.

Heterozygous:
Conditional mouse model; no phenotype unless flox-stop-flox-cassette is deleted by cre-mediated recombination.
Breeding historyBackcrossing to C57BL/6N for about 5-6 generations, followed by intercrosses.
References
  • The miR-26 family regulates early B cell development and transformation.;Hutter Katharina, Lindner Silke E, Kurschat Constanze, Rülicke Thomas, Villunger Andreas, Herzog Sebastian, ;2022;Life science alliance;5;; 35459737
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreUniversity of Veterinary Medicine, Vienna, Austria
Animals used for archivinghomozygous C57BL/6 x 129/Sv males

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (gene matching)
  • abnormal adipose tissue distribution / MGI
  • big ears / MGI
  • delayed bone ossification / MGI
  • fragile skeleton / MGI
  • increased bone mineral density / MGI
  • decreased bone mineral density / MGI
  • failure of bone resorption / MGI
  • osteopetrosis / MGI
  • kyphoscoliosis / MGI
  • abnormal neurocranium morphology / MGI
  • decreased compact bone thickness / MGI
  • abnormal vertebral body morphology / MGI
  • abnormal rib morphology / MGI
  • kyphosis / MGI
  • scoliosis / MGI
  • heart right ventricle hypertrophy / MGI
  • increased cell proliferation / MGI
  • abnormal cell morphology / MGI
  • focal hair loss / MGI
  • abnormal cranium morphology / MGI
  • abnormal maxilla morphology / MGI
  • abnormal digestive system morphology / MGI
  • abnormal forelimb morphology / MGI
  • abnormal femur morphology / MGI
  • syndactyly / MGI
  • oligodactyly / MGI
  • abnormal autopod morphology / MGI
  • thin tail / MGI
  • abnormal liver morphology / MGI
  • weakness / MGI
  • hindlimb paralysis / MGI
  • abnormal uterus morphology / MGI
  • lung hemorrhage / MGI
  • thick skin / MGI
  • skin hyperplasia / MGI
  • abnormal dermal layer morphology / MGI
  • thick dermal layer / MGI
  • increased body length / MGI
  • decreased body length / MGI
  • increased body weight / MGI
  • decreased body weight / MGI
  • increased body size / MGI
  • decreased body size / MGI
  • abnormal tail movements / MGI
  • abnormal locomotor behavior / MGI
  • hypoactivity / MGI
  • abnormal gait / MGI
  • abnormal motor coordination/balance / MGI
  • abnormal osteoclast physiology / MGI
  • abnormal cardiovascular system physiology / MGI
  • abnormal blood vessel morphology / MGI
  • embryonic growth arrest / MGI
  • lung inflammation / MGI
  • hemorrhage / MGI
  • intracranial hemorrhage / MGI
  • increased litter size / MGI
  • decreased litter size / MGI
  • delayed sexual maturation / MGI
  • respiratory distress / MGI
  • abnormal coat/hair pigmentation / MGI
  • premature death / MGI
  • abnormal limb morphology / MGI
  • abnormal digit morphology / MGI
  • abnormal limb bone morphology / MGI
  • abnormal fertility/fecundity / MGI
  • no abnormal phenotype detected / MGI
  • abnormal fibula morphology / MGI
  • abnormal turbinate morphology / MGI
  • decreased susceptibility to hepatic steatosis / MGI
  • abnormal blood gas level / MGI
  • delayed vaginal opening / MGI
  • decreased circulating insulin level / MGI
  • abnormal mechanical nociception / MGI
  • decreased vertical activity / MGI
  • abnormal dental pulp cavity morphology / MGI
  • coarse hair / MGI
  • increased growth rate / MGI
  • increased insulin sensitivity / MGI
  • abnormal bone mineralization / MGI
  • increased circulating parathyroid hormone level / MGI
  • decreased circulating alkaline phosphatase level / MGI
  • arthritis / MGI
  • abnormal bone healing / MGI
  • no phenotypic analysis / MGI
  • decreased skin tensile strength / MGI
  • decreased tendon stiffness / MGI
  • short femur / MGI
  • decreased susceptibility to age related obesity / MGI
  • aneurysm / MGI
  • osteoarthritis / MGI
  • abnormal long bone morphology / MGI
  • abnormal compact bone morphology / MGI
  • abnormal forelimb zeugopod morphology / MGI
  • increased hepatocyte proliferation / MGI
  • abnormal hormone level / MGI
  • increased lean body mass / MGI
  • decreased thermal nociceptive threshold / MGI
  • decreased bone mass / MGI
  • aortic dissection / MGI
  • abnormal chest morphology / MGI
  • abnormal pubis morphology / MGI
  • abnormal ischium morphology / MGI
  • abnormal pelvic girdle bone morphology / MGI
  • thin ribs / MGI
  • rib fractures / MGI
  • decreased length of long bones / MGI
  • abnormal vertebral column morphology / MGI
  • short vertebral body / MGI
  • increased susceptibility to age-related hearing loss / MGI
  • abnormal osteoblast morphology / MGI
  • increased osteoblast cell number / MGI
  • decreased bone strength / MGI
  • increased bone resorption / MGI
  • abnormal osteoblast physiology / MGI
  • liver hyperplasia / MGI
  • decreased susceptibility to injury / MGI
  • ocular hypertension / MGI
  • improved glucose tolerance / MGI
  • abnormal humerus morphology / MGI
  • abnormal enzyme/coenzyme level / MGI
  • abnormal myocardium layer morphology / MGI
  • homeostasis/metabolism phenotype / MGI
  • hearing/vestibular/ear phenotype / MGI
  • digestive/alimentary phenotype / MGI
  • reproductive system phenotype / MGI
  • skeleton phenotype / MGI
  • vision/eye phenotype / MGI
  • decreased percent body fat/body weight / MGI
  • abnormal skeleton morphology / MGI
  • decreased circulating glucose level / MGI
  • decreased ventricle muscle contractility / MGI
  • decreased susceptibility to diet-induced obesity / MGI
  • spinal hemorrhage / MGI
  • abnormal heart septum morphology / MGI
  • impaired hearing / MGI
  • decreased diameter of femur / MGI
  • abnormal myometrium morphology / MGI
  • abnormal osteoblast differentiation / MGI
  • abnormal cutaneous collagen fibril morphology / MGI
  • abnormal osteocyte morphology / MGI
  • fused carpal bones / MGI
  • delayed female fertility / MGI
  • small pancreatic islets / MGI
  • decreased trabecular bone thickness / MGI
  • abnormal endoplasmic reticulum morphology / MGI
  • abnormal aorta elastic tissue morphology / MGI
  • abnormal aorta tunica adventitia morphology / MGI
  • decreased total body fat amount / MGI
  • abnormal thoracic cage shape / MGI
  • decreased bone mineral content / MGI
  • abnormal heart electrocardiography waveform feature / MGI
  • myocardial hypertrophy / MGI
  • increased QRS amplitude / MGI
  • abnormal bone trabecula morphology / MGI
  • decreased bone trabecula number / MGI
  • decreased bone volume / MGI
  • decreased trabecular bone volume / MGI
  • pulmonary alveolar hemorrhage / MGI
  • abnormal compact bone lamellar structure / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • neonatal lethality, complete penetrance / MGI
  • neonatal lethality, incomplete penetrance / MGI
  • prenatal lethality, complete penetrance / MGI
  • embryonic lethality, complete penetrance / MGI
  • embryonic lethality during organogenesis, complete penetrance / MGI
  • preweaning lethality, incomplete penetrance / MGI
  • abnormal bone collagen fibril morphology / MGI
  • abnormal tendon collagen fibril morphology / MGI
  • decreased neuronal stem cell self-renewal / MGI
  • thin body / MGI
  • abnormal extracellular matrix morphology / MGI
  • osteophytes / MGI
  • increased bone trabecular spacing / MGI
  • decreased bone trabecular spacing / MGI
  • decreased bone ossification / MGI
  • decreased bone mineralization / MGI
  • decreased collagen level / MGI
  • broad face / MGI
  • thick neurocranium / MGI
  • thin neurocranium / MGI
  • increased osteoblast apoptosis / MGI
  • abnormal dentin mineralization / MGI
  • increased osteocyte apoptosis / MGI
  • abnormal osteocyte lacuna morphology / MGI
  • joint contracture / MGI
  • atlanto-occipital joint dislocation / MGI
  • abnormal olecranon morphology / MGI

Literature references

  • The miR-26 family regulates early B cell development and transformation.;Hutter Katharina, Lindner Silke E, Kurschat Constanze, Rülicke Thomas, Villunger Andreas, Herzog Sebastian, ;2022;Life science alliance;5;; 35459737

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

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Practical information

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