B6;129-Prdm8^tm1Mci/H

Status	Available to order
EMMA ID	EM:12207
Citation information	RRID:IMSR_EM:12207 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6;129-Prdm8^tm1Mci/H
Alternative name	Prdm8-eGFP knock in (Background=50%C57BL/6J; 25% 129S1/SviMJ; 25% 129X1/SvJ)
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Prdm8^tm1Mci
Gene/Transgene symbol	Prdm8

Information from provider

Provider	Rod McInnes
Provider affiliation	Lady Davis Research Institute, McGill University
Additional owner	Prof. Rod McInnes, Lady Davis Research Institute, Montreal, Canada
Genetic information	GFP knock-in replacing the second coding exon and part of the third coding exon of the Prdm8 gene, using an IRES-EGFPnls/DTA-Neo reporter/selection cassette.
Phenotypic information	Homozygous: Homozygous mice have a night blindness phenotype due to the loss of retinal rod bipolar cells (Jung, Atan et al. PNAS 2015). Heterozygous: Heterozygous mice are identical to wild-type.
Breeding history	Maintained on mixed background (50% C57BL/6J; 25% 129S1/SviMJ; 25% 129X1/SvJ).
References	Transcription factor PRDM8 is required for rod bipolar and type 2 OFF-cone bipolar cell survival and amacrine subtype identity.;Jung Cynthia C, Atan Denize, Ng David, Ploder Lynda, Ross Sarah E, Klein Martin, Birch David G, Diez Eduardo, McInnes Roderick R, ;2015;Proceedings of the National Academy of Sciences of the United States of America;112;E3010-9; 26023183 Bhlhb5 and Prdm8 form a repressor complex involved in neuronal circuit assembly.;Ross Sarah E, McCord Alejandra E, Jung Cynthia, Atan Denize, Mok Stephanie I, Hemberg Martin, Kim Tae-Kyung, Salogiannis John, Hu Linda, Cohen Sonia, Lin Yingxi, Harrar Dana, McInnes Roderick R, Greenberg Michael E, ;2012;Neuron;73;292-303; 22284184
Homozygous fertile	no
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	not known

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Early-onset Lafora body disease / Orphanet_324290

IMPC phenotypes (gene matching)

increased heart rate / IMPC
increased circulating sodium level / IMPC
abnormal thymus morphology / IMPC
enlarged thymus / IMPC
abnormal retina morphology / IMPC
hyperactivity / IMPC
impaired glucose tolerance / IMPC
decreased locomotor activity / IMPC
abnormal tooth morphology / IMPC
decreased body weight / IMPC
increased prepulse inhibition / IMPC
increased freezing behavior / IMPC
abnormal spleen morphology / IMPC
abnormal skin morphology / IMPC
abnormal seminal vesicle morphology / IMPC
abnormal heart left ventricle morphology / IMPC
increased airway resistance / IMPC
small heart / IMPC
small spleen / IMPC
abnormal inspiratory capacity / IMPC
abnormal uterus morphology / IMPC
abnormal heart morphology / IMPC
increased anxiety-related response / IMPC
enlarged uterus / IMPC
increased lung elastance / IMPC

MGI phenotypes (gene matching)

skin lesions / MGI
excessive scratching / MGI
abnormal motor coordination/balance / MGI
absent corpus callosum / MGI
abnormal axon guidance / MGI
nervous system phenotype / MGI
absent hippocampal commissure / MGI

Literature references

Transcription factor PRDM8 is required for rod bipolar and type 2 OFF-cone bipolar cell survival and amacrine subtype identity.;Jung Cynthia C, Atan Denize, Ng David, Ploder Lynda, Ross Sarah E, Klein Martin, Birch David G, Diez Eduardo, McInnes Roderick R, ;2015;Proceedings of the National Academy of Sciences of the United States of America;112;E3010-9; 26023183
Bhlhb5 and Prdm8 form a repressor complex involved in neuronal circuit assembly.;Ross Sarah E, McCord Alejandra E, Jung Cynthia, Atan Denize, Mok Stephanie I, Hemberg Martin, Kim Tae-Kyung, Salogiannis John, Hu Linda, Cohen Sonia, Lin Yingxi, Harrar Dana, McInnes Roderick R, Greenberg Michael E, ;2012;Neuron;73;292-303; 22284184

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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B6;129-Prdm8tm1Mci/H