C57BL/6NCrl-Ddi2^{tm1b(EUCOMM)Hmgu}/Ph

Status	Available to order
EMMA ID	EM:12340
Citation information	RRID:IMSR_EM:12340 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6NCrl-Ddi2^{tm1b(EUCOMM)Hmgu}/Ph
Alternative name	HEPD0660_5_E02
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Ddi2^{tm1b(EUCOMM)Hmgu}
Gene/Transgene symbol	Ddi2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Institute of Molecular Genetics
Provider affiliation	Department of Transgenic Models of Diseases, Institute of Molecular Genetics
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0660_5_E02. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele (Gt(ROSA)26Sortm1(ACTB-cre,-EGFP)Ics (MGI:5285392)). Click here for more information on EUCOMM final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	DDI2 protease controls embryonic development and inflammation via TCF11/NRF1.;Nedomova Monika, Haberecht-Müller Stefanie, Möller Sophie, Venz Simone, Prochazkova Michaela, Prochazka Jan, Sedlak Frantisek, Chawengsaksophak Kallayanee, Hammer Elke, Kasparek Petr, Adamek Michael, Sedlacek Radislav, Konvalinka Jan, Krüger Elke, Grantz Saskova Klara, ;2024;iScience;27;110893; 39328932

Information from EMMA

Archiving centre	Institute of Molecular Genetics, Prague, Czech Republic
Animals used for archiving	heterozygous C57BL/6NCrl males

Disease and phenotype information

IMPC phenotypes (allele matching)

abnormal urinary bladder morphology / IMPC
abnormal spine curvature / IMPC
abnormal auditory brainstem response / IMPC
impaired glucose tolerance / IMPC
increased airway resistance / IMPC
preweaning lethality, complete penetrance / IMPC
prenatal lethality prior to heart atrial septation / IMPC

IMPC phenotypes (gene matching)

impaired glucose tolerance / IMPC
abnormal auditory brainstem response / IMPC
preweaning lethality, complete penetrance / IMPC
prenatal lethality prior to heart atrial septation / IMPC
abnormal urinary bladder morphology / IMPC
increased airway resistance / IMPC
abnormal spine curvature / IMPC

Literature references

DDI2 protease controls embryonic development and inflammation via TCF11/NRF1.;Nedomova Monika, Haberecht-Müller Stefanie, Möller Sophie, Venz Simone, Prochazkova Michaela, Prochazka Jan, Sedlak Frantisek, Chawengsaksophak Kallayanee, Hammer Elke, Kasparek Petr, Adamek Michael, Sedlacek Radislav, Konvalinka Jan, Krüger Elke, Grantz Saskova Klara, ;2024;iScience;27;110893; 39328932

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6NCrl-Ddi2tm1b(EUCOMM)Hmgu/Ph