B6;129(Cg)-Pcbp4tm1.1Geno/Cnbc
| Status | Available to order |
| EMMA ID | EM:12611 |
| Citation information | RRID:IMSR_EM:12611 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;129(Cg)-Pcbp4tm1.1Geno/Cnbc |
| Alternative name | PCBP4 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Pcbp4tm1.1Geno |
| Gene/Transgene symbol | Pcbp4 |
Information from provider
| Provider | Ruben Pio |
| Provider affiliation | FIMA |
| Additional owner | genOway, France |
| Genetic information | This is a knockout mouse line with a targeted inactivation of Pcbp4 gene. This mouse gene is located on chromosome 9 and extends on around 10 kb. The gene is composed of 13 exons. The cDNA contains 1984 nucleotides. The open reading frame encodes for a 403 amino acid polypeptide. The translation initiation site is located in exon 2 and the stop codon in exon 13. The constitutive knockout line was generated using a conditional gene targeting strategy (“Safe knock-out” approach from GenOway). Using homologous recombination, the targeted region (exons 2 to 12 containing the 3 KH functional domains of the PCBP4 protein) was flanked by two loxP sites. Targeted GenOway 129/SvPas ES clones were generated and injected into C57BL/6 blastocysts. Chimeras were crossed with C57BL/6 CMV-cre mice to induce a heterozygous deletion of Pcbp4. The animals were intercrossed to generated homozygous Pcbp4 KO mice. |
| Phenotypic information | Homozygous:Viable. No observed phenotypic variations.Heterozygous:Viable. No observed phenotypic variations. |
| Breeding history | The line has been maintained by intercrossing C57BL/6 homozygous mice. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6J males |