B6.129P2-Dlg1tm1.1Abrie/Orl
| Status | Available to order |
| EMMA ID | EM:12717 |
| Citation information | RRID:IMSR_EM:12717 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129P2-Dlg1tm1.1Abrie/Orl |
| Alternative name | Dlg1tm1.1Abrie |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Dlg1tm1.1Abrie |
| Gene/Transgene symbol | Dlg1 |
Information from provider
| Provider | Hugues Abriel |
| Provider affiliation | IBMM |
| Genetic information | Dlg1 (SAP97) gene is dispersed over 26 exons (25 coding) in a genomic region of 110 kbp on chromosome 16. Dlg proteins are involved in cell proliferation, synapse formation and function, and epithelial cell polarity. The phenotype of a constitutive SAP97 gene trap mutant includes craniofacial abnormalities, neonatal lethality, and a number of malformations predominantly in the kidney and in the eye (Caruana, G. and Bernstein, A. (2001), Mol Cell Biol 21(5):1475-83; Naim, E. et al. (2005), Kidney Int 68(3):955-65; Nguyen, M.M. et al. (2003), Mol Cell Biol 23(24):8970-81). We aimed at deleting SAP97 in somatic cells upon induction in the heart using a cre recomb.-expressing specific mouse line, so we flanked SAP97 exons 1-3, including 900 bp of the upstream sequence, with loxP sites. Analysing the upstream region of SAP97 we identified a strong TATA box consensus preceded by a T-rich stretch about 130 bp upstream of the putative exon 1 (as there are, to our knowledge, no promoter studies, the exact transcription start site has not been mapped). Deletion of the loxP flanked region should therefore lead to the loss of the putative core promoter and the first three exons of SAP97 resulting in the loss of SAP97. |
| Phenotypic information | Homozygous:No phenotypeHeterozygous:No phenotype |
| Breeding history | Mice were bred to obtain a floxed homozygous genotype and then more than 10 backcrosses to C57BL/6J. At least once every 1-2 years a new step of backcrossing has been done to refresh the line. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | homozygous C57BL/6J males |
Literature references
- Cardiac-specific ablation of synapse-associated protein SAP97 in mice decreases potassium currents but not sodium current.;Gillet Ludovic, Rougier Jean-Sébastien, Shy Diana, Sonntag Stephan, Mougenot Nathalie, Essers Maria, Shmerling Doron, Balse Elise, Hatem Stéphane N, Abriel Hugues, ;2015;Heart rhythm;12;181-92; 25447080