B6.129P2(Cg)-Snta1tm1.1Pg/Orl
| Status | Available to order |
| EMMA ID | EM:12718 |
| Citation information | RRID:IMSR_EM:12718 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129P2(Cg)-Snta1tm1.1Pg/Orl |
| Alternative name | Snta1 tm1Pg |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Snta1tm1.1Pg |
| Gene/Transgene symbol | Snta1 |
Information from provider
| Provider | Hugues Abriel |
| Provider affiliation | IBMM |
| Genetic information | To study the function of alpha1-syntrophin (Snta1), a conditional mouse model was generated that allows tissue or time-point specific inactivation of the Snta1 gene by the use of the cre recombinase/loxP system. The design of the mouse model is based on a modified Snta1 locus with two loxP sites flanking part of Snta1 exon 1. One loxP site is inserted in the 5-prime UTR in exon 1, whereas the second loxP site is located in intron 1. For the introduction of loxP sites into the Snta1 locus, a targeting vector (pSnta1 TV) was generated that contained homology regions of 2.2 kb (short homology region) and 5.6 kb (long homology region). The 3-prime loxP site was inserted together with an FRT-flanked neomycin cassette into a KpnI restriction site 269 bp downstream of Snta1 exon 1. The 5-prime loxP site was inserted 50 bp downstream of the start of exon 1 and 78 bp upstream of the ATG start codon in the 5-prime untranslated region. In total, a region of 639 bp was flanked by loxP sites, containing the coding part of Snta1 exon 1. |
| Phenotypic information | Homozygous:No phenotypeHeterozygous:No phenotype |
| Breeding history | Male mice with a high degree of coat color chimerism were mated to flp-deleter mice (C57BL/6 background) to generate heterozygous Snta1-floxed mice. Mice were bred to obtain a homozygous floxed genotype, followed by more than 10 backcrosses to C57BL/6 background. Once every 1-2 years a new step of backcrossing is performed to refresh the line. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | homozygous C57BL/6J males |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Romano-Ward syndrome / Orphanet_101016
Literature references
- A Distinct Pool of Nav1.5 Channels at the Lateral Membrane of Murine Ventricular Cardiomyocytes.;Rougier Jean-Sébastien, Essers Maria C, Gillet Ludovic, Guichard Sabrina, Sonntag Stephan, Shmerling Doron, Abriel Hugues, ;2019;Frontiers in physiology;10;834; 31333492