STOCK Gsk3atm1Dral Gsk3btm1Dral/H

Status

Available to order

EMMA IDEM:12809
Citation informationRRID:IMSR_EM:12809 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameSTOCK Gsk3atm1Dral Gsk3btm1Dral/H
Alternative nameGsk3a/b 21A/21A/9A/9A
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolGsk3atm1Dral, Gsk3btm1Dral
Gene/Transgene symbolGsk3a, Gsk3b

Information from provider

ProviderGail Gilmour
Provider affiliationMRC PPU, School of Life Sciences, University of Dundee
Genetic informationReplacement of N-terminal phosphorylation site serine with non-phosphorylatable alanine on both GSK3 isoforms. This mutation disrupts key inhibitory phosphorylation site in both GSK alpha and GSK3 beta preventing it from becoming inactivated by Akt and other AGC kinases. In these mice insulin can no longer induce activation of glycogen synthase. These animals are excellent models to study the role of inactivation of GSK3 isoforms in biology.
Phenotypic informationHomozygous:
No adverse phenotype. Can be maintained by hom x hom mating.

Heterozygous:
No adverse phenotype.
Breeding historyUnknown breeding history as sourced from another facility. Rederived at MRC Harwell on a C57BL/6J background before submission to EMMA Repository.
References
  • Role that phosphorylation of GSK3 plays in insulin and Wnt signalling defined by knockin analysis.;McManus Edward J, Sakamoto Kei, Armit Laura J, Ronaldson Leah, Shpiro Natalia, Marquez Rodolfo, Alessi Dario R, ;2005;The EMBO journal;24;1571-83; 15791206
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)
  • abnormal snout morphology / IMPC
  • decreased circulating fructosamine level / IMPC
  • improved glucose tolerance / IMPC
  • decreased mean platelet volume / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • prolonged QRS complex duration / IMPC
  • abnormal gait / IMPC
  • decreased startle reflex / IMPC
  • decreased body weight / IMPC
  • decreased total body fat amount / IMPC
  • increased lean body mass / IMPC
  • decreased body length / IMPC
  • decreased exploration in new environment / IMPC
  • decreased circulating glucose level / IMPC
  • decreased lean body mass / IMPC
MGI phenotypes (gene matching)
  • delayed bone ossification / MGI
  • cleft palate / MGI
  • abnormal liver morphology / MGI
  • abnormal hepatocyte morphology / MGI
  • decreased exploration in new environment / MGI
  • abnormal contextual conditioning behavior / MGI
  • abnormal skeleton physiology / MGI
  • abnormal learning/memory/conditioning / MGI
  • abnormal glucose homeostasis / MGI
  • abnormal skeleton development / MGI
  • no abnormal phenotype detected / MGI
  • behavioral despair / MGI
  • decreased circulating insulin level / MGI
  • abnormal bone healing / MGI
  • no phenotypic analysis / MGI
  • increased myocardial infarction size / MGI
  • decreased myocardial infarction size / MGI
  • increased compact bone thickness / MGI
  • split sternum / MGI
  • xiphoid process foramen / MGI
  • increased bone resorption / MGI
  • abnormal osteoblast physiology / MGI
  • homeostasis/metabolism phenotype / MGI
  • behavior/neurological phenotype / MGI
  • abnormal glycogen homeostasis / MGI
  • decreased circulating glucose level / MGI
  • increased bone mass / MGI
  • abnormal sternum ossification / MGI
  • abnormal spatial reference memory / MGI
  • decreased prepulse inhibition / MGI
  • abnormal palate development / MGI
  • abnormal palatal shelf elevation / MGI
  • palatal shelves fail to meet at midline / MGI
  • cleft secondary palate / MGI
  • abnormal Wallerian degeneration / MGI
  • increased trabecular bone mass / MGI
  • increased trabecular bone volume / MGI
  • increased compact bone mass / MGI
  • increased compact bone volume / MGI
  • neonatal lethality, complete penetrance / MGI
  • lethality throughout fetal growth and development, complete penetrance / MGI
  • preweaning lethality, complete penetrance / MGI
  • enhanced osteoblast differentiation / MGI
  • increased bone ossification / MGI

Literature references

  • Role that phosphorylation of GSK3 plays in insulin and Wnt signalling defined by knockin analysis.;McManus Edward J, Sakamoto Kei, Armit Laura J, Ronaldson Leah, Shpiro Natalia, Marquez Rodolfo, Alessi Dario R, ;2005;The EMBO journal;24;1571-83; 15791206

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

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