IMPC phenotypes (gene matching)
- preweaning lethality, incomplete penetrance / IMPC
STOCK Loxl2tm1.2Acan/Cnbc
| Status | Available to order |
| EMMA ID | EM:12882 |
| Citation information | RRID:IMSR_EM:12882 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | STOCK Loxl2tm1.2Acan/Cnbc |
| Alternative name | CFB |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Loxl2tm1.2Acan |
| Gene/Transgene symbol | Loxl2 |
Information from provider
| Provider | Amparo Cano |
| Provider affiliation | Biochemistry, Autonoma University (Medicine Faculty) |
| Additional owner | Francisco Portillo and Alberto Martin, Universidad Autónoma de Madrid, Spain. |
| Genetic information | Constitutive Loxl2 knock-out. A conditional knock-out mutation of Loxl2 based on cre recombinase/loxP system was introduced by homologous recombination in the endogenous locus of Loxl2. |
| Phenotypic information | Homozygous:Partial perinatal lethality due to cardiac and liver defects. Knock-out mice develop a lower number of skin tumors after being subjected to DMBA/TPA (dimethylbenz-anthracene and 12-O-tetradecanoyl phorbol-13-acetate) treatment.Heterozygous:Completely normal |
| Breeding history | ES cell clones positive for the mutation were microinjected/aggregated into receptive blastocysts/morulas and chimeras were obtained. These animals were crossed with cre recombinase-positive mice in order to check germline transmission and, at the same time, obtain animals harbouring a constitutive knock-out allele. The resultant heterozygous animals were intercrossed and knock-out animals were obtained. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous mixed 129S/SvEv and C57BL/6 and FVB, N5 C57BL/6 males, wild-type C57BL/6J females |
| Stage of embryos | 2-cell |
Disease and phenotype information
Literature references
- Lysyl oxidase-like 2 represses Notch1 expression in the skin to promote squamous cell carcinoma progression.;Martin Alberto, Salvador Fernando, Moreno-Bueno Gema, Floristán Alfredo, Ruiz-Herguido Cristina, Cuevas Eva P, Morales Saleta, Santos Vanesa, Csiszar Katalin, Dubus Pierre, Haigh Jody J, Bigas Anna, Portillo Francisco, Cano Amparo, ;2015;The EMBO journal;34;1090-109; 25759215
- Loxl2 and Loxl3 Paralogues Play Redundant Roles during Mouse Development.;Santamaría Patricia G, Dubus Pierre, Bustos-Tauler José, Floristán Alfredo, Vázquez-Naharro Alberto, Morales Saleta, Cano Amparo, Portillo Francisco, ;2022;International journal of molecular sciences;23;; 35628534